Related Experiment Video
Updated: Aug 14, 2026

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells
Published on: December 17, 2015
Determining Histone Posttranslational Modifications Along the Cell Cycle in Trypanosoma cruzi by Flow Cytometry
María Del Rosario López1, Guillermo D Alonso2,3, Josefina Ocampo4
1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"- Consejo Nacional de Investigaciones Científicas y Técnicas (INGEBI-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
Abstract:
Detecting histone posttranslational modifications is important to understand the histone code in eukaryotic organisms. Here, we describe the use of flow cytometry to detect these epigenetic marks along the cell cycle in Trypanosoma cruzi. Using fluorescently labeled antibodies directed against specific histone variants or histone posttranslational modifications, flow cytometry allows the simultaneous detection of several parameters on a cell-to-cell basis.

