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In vitro treatment of oral cancer cells using copper and glucose oxidase-based nanoparticles
Hyeong Sun Park1, Unshul Shin1, So-Young Park2
1Department of Dental Materials, School of Dentistry, Pusan National University, Yangsan, Korea.
Purpose:
To test the in vitro effectiveness of GOx-combined nanoparticles (pvp-Cu-GOx) in treating oral cancer cells under hydrogen peroxide and laser irradiation conditions.
Methods:
Using polymer, metal, and glucose oxidase, nanoparticles were synthesized. Their ability to kill cancer cells was evaluated through an in vitro cell viability test. Methylene blue (MB) degradation test and DPBF assay were performed to evaluate ROS generation. Oxygen production and glucose consumption were also evaluated.
Results:
GOx-combined nanoparticles (pvp-Cu-GOx) achieved cancer cell death over 80% in 100 µM H₂O₂. Much degradation of MB solution occurred by pvp-Cu-GOx compared to that of pvp-Cu. Oxygen was produced up to 4.3 mg/L by pvp-Cu-GOx at 200 ppm concentration. pvp-Cu-GOx consumed glucose though not high compared to that by glucose oxidase itself. The obtained results are the combined reactions of ROS and glucose oxidase.
Clinical Significance:
GOx-combined nanoparticles pvp-Cu-GOx can significantly lower in vitro cancer cell viability in 100 µM H₂O₂. pvp-Cu-GOx has great potential to be used in clinical applications after passing in vivo verification.
