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Related Experiment Video

Updated: Jan 12, 2026

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis

Published on: October 14, 2025

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis.

Bing Zhou¹, Ruixuan Liu², Jiaxin Sun³

¹Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, Beihang University; Interdisciplinary Innovation Institute of Medicine and Engineering, Beihang University.

Journal of Visualized Experiments : Jove

|November 3, 2025

Summary

Researchers developed a microfluidic platform to study neuronal metabolism after axonal injury. This system preserves in vivo metabolic features, aiding research into neurodegeneration and axon regeneration.

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Last Updated: Jan 12, 2026

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis

Published on: October 14, 2025

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

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Published on: February 7, 2014

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

Published on: September 10, 2009

Area of Science:

Neuroscience
Cellular Metabolism
Biotechnology

Background:

Neurons require axonal homeostasis for regeneration and to prevent neurodegeneration.
Neuronal metabolism is compartmentalized and influenced by the microenvironment, complicating in vivo studies.
Understanding intrinsic metabolic mechanisms post-axonal injury is crucial.

Purpose of the Study:

To develop a microfluidic platform for studying neuronal metabolism after axonal injury.
To create a simplified in vitro model that preserves in vivo metabolic characteristics.
To enable high-throughput multi-omics analyses of neuronal metabolic remodeling.

Main Methods:

Developed a microfluidic platform with separate soma and axon chambers connected by microchannels.
Induced axonal injury via vacuum aspiration in the axon compartment.
Designed high-throughput chips for large-scale transcriptomic and metabolomic analyses.

Main Results:

The platform successfully cultured primary cortical neurons, preserving physiological glycolytic flux and mitochondrial respiration.
It provides a simplified model for investigating intrinsic metabolic changes following axonal injury.
The system allows for rapid assessment of metabolite and enzyme dynamics, enhancing multi-omics accuracy.

Conclusions:

The developed microfluidic platform effectively models neuronal metabolic responses to axonal injury in vitro.
This tool facilitates deeper investigation into axon regeneration and neurodegeneration mechanisms.
It offers a reproducible and accurate method for multi-omics studies in neuroscience.