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Related Experiment Video

Updated: Jun 27, 2026

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments

Published on: January 27, 2016

Z-DNA interaction proteins - insights from ChIP-seq data.

Michaela Dobrovolná¹, Václav Brázda¹

¹Institute of Biophysics, Czech Academy of Sciences, Královopolská 135, 612 00, Brno, Czech Republic; Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00, Brno, Czech Republic.

Biochemical and Biophysical Research Communications

|November 3, 2025

Summary

This summary is machine-generated.

Researchers identified new Z-DNA-binding proteins (ZBPs) in the human genome using the Z-Hunter algorithm and ChIP-Atlas data. This discovery expands our understanding of Z-DNA

Keywords:

Bioinformatics ChIP-atlas ChIP-seq Gene regulation Transcription factor Z-DNA

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Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

Z-DNA is a left-handed DNA structure involved in gene regulation.
The full range of Z-DNA-binding proteins (ZBPs) in humans is not well understood.

Purpose of the Study:

To identify Z-DNA-binding proteins (ZBPs) in the human genome.
To explore the role of Z-DNA in transcriptional regulation.

Main Methods:

Utilized the Z-Hunter algorithm to predict Z-DNA-forming sequences genome-wide.
Integrated predicted Z-DNA regions with transcription factor (TF) binding data from ChIP-Atlas.
Identified proteins co-localizing with Z-DNA motifs.

Main Results:

Discovered a wide array of candidate ZBPs, including known homologs and novel transcription factors.
Demonstrated potential co-localization between Z-DNA motifs and TF binding sites.
Suggested an expanded role for Z-DNA in controlling gene expression.

Conclusions:

The study provides a comprehensive map of potential Z-DNA-protein interactions.
Identified ZBPs may play a significant role in human health and disease.
This work serves as a foundation for further research into Z-DNA regulatory networks.