Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

4.4K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
4.4K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

6.0K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
6.0K
Cell Migration01:09

Cell Migration

18.5K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.5K
Cell Migration01:19

Cell Migration

6.4K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.4K
Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.9K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.9K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.4K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two-pore channel-2 controls calmodulin-dependent STIM1 inactivation.

Nature communications·2026
Same author

Pathogenic Gαo Mutants Drive Dominant GPCR Coupling in GNAO1 Encephalopathies.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

STIM1/2 maintain signaling competence at ER-PM contact sites during neutrophil spreading.

The Journal of cell biology·2025
Same author

Disparate molecular mechanisms in cardiac ryanodine receptor channelopathies.

Frontiers in molecular biosciences·2025
Same author

S-acylation of Ca<sup>2+</sup> transport proteins in cancer.

Chronic diseases and translational medicine·2024
Same author

S-acylation of Ca2+ transport proteins: molecular basis and functional consequences.

Biochemical Society transactions·2024

Related Experiment Video

Updated: Jan 12, 2026

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
15:01

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

Published on: September 24, 2011

20.1K

Calcium Signaling in Migrating Neutrophils.

Camille Rabesahala de Meritens1, Nicolas Demaurex2

  • 1Department of Cell Physiology and Metabolism, University of Geneva, Geneva 1211, Switzerland.

Cold Spring Harbor Perspectives in Biology
|November 3, 2025
PubMed
Summary
This summary is machine-generated.

Calcium (Ca2+) signals are crucial for neutrophil function, enhancing their ability to fight infections. This review explores how Ca2+ regulates neutrophil adhesion, spreading, and migration through selectin and integrin pathways.

More Related Videos

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions
08:41

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Published on: May 10, 2022

1.8K
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
11:26

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Published on: August 14, 2014

20.0K

Related Experiment Videos

Last Updated: Jan 12, 2026

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy
15:01

Tracking Neutrophil Intraluminal Crawling, Transendothelial Migration and Chemotaxis in Tissue by Intravital Video Microscopy

Published on: September 24, 2011

20.1K
Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions
08:41

Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

Published on: May 10, 2022

1.8K
Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow
11:26

Real-time Imaging of Endothelial Cell-cell Junctions During Neutrophil Transmigration Under Physiological Flow

Published on: August 14, 2014

20.0K

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Neutrophils are key white blood cells combating bacterial and fungal infections.
  • Cytosolic calcium (Ca2+) elevations boost neutrophil microbicidal activity.
  • The precise role of Ca2+ in neutrophil adhesion, spreading, and trans-endothelial migration remains incompletely understood.

Purpose of the Study:

  • To review the regulatory mechanisms of Ca2+ signaling in neutrophil adhesion and spreading.
  • To elucidate the molecular and ultrastructural basis of localized Ca2+ signals within neutrophils.
  • To explore signaling pathways that decode Ca2+ signals sustaining neutrophil motility.

Main Methods:

  • Literature review of existing research on neutrophil function and calcium signaling.
  • Analysis of molecular and cellular mechanisms underlying neutrophil migration.
  • Synthesis of data on selectin and integrin-mediated signaling pathways.

Main Results:

  • Selectin and integrin engagement triggers Ca2+ elevations that are critical for neutrophil adhesion and spreading.
  • Localized Ca2+ signals play a significant role in regulating neutrophil behavior.
  • Specific pathways decode Ca2+ signals to sustain actin-based neutrophil motility.

Conclusions:

  • Calcium signaling is a central regulator of neutrophil adhesion, spreading, and migration.
  • Understanding Ca2+ dynamics provides insights into neutrophil's role in immunity and inflammation.
  • Further research into Ca2+ decoding pathways can reveal therapeutic targets for inflammatory diseases.