The SlMYC2-SlNAC90-SlNAC102 Module Mediates Low-Temperature Stress Responses in Tomato by Regulating Jasmonic Acid...

Low-temperature stress is a prevalent abiotic factor that severely restricts plant growth and development. NAC transcription factors play a critical role in plant adaptation to various abiotic stresses. In this study, we demonstrate that SlNAC90 regulates the expression of SlLOXC in tomato through direct binding, promoting the biosynthesis of jasmonic acid (JA) and positively modulating the plant's tolerance to low-temperature stress. Overexpression of SlLOXC significantly enhances JA accumulation under low-temperature stress and reduces the excessive accumulation of reactive oxygen species (ROS), whereas its knockout results in an opposing phenotype. Further investigations reveal that SlNAC90 interacts with SlNAC102 at the protein level, and the binding between the two enhances SlNAC90's regulatory effect on SlLOXC. In addition, SlMYC2, a key transcription factor in the JA signalling pathway, can directly bind to both SlNAC90 and SlNAC102, promoting their expression. This study uncovers the central role of the SlMYC2-SlNAC90-SlNAC102-SlLOXC module in regulating JA biosynthesis and clarifies the molecular mechanism by which this module participates in the response of tomato to low-temperature stress by modulating JA accumulation.