The SlMYC2-SlNAC90-SlNAC102 Module Mediates Low-Temperature Stress Responses in Tomato by Regulating Jasmonic Acid...

Area of Science:

• Plant Biology
• Molecular Biology
• Stress Physiology

Background:

• Low-temperature stress significantly hinders plant growth and development.
• NAC transcription factors are vital for plant adaptation to abiotic stresses.

Purpose of the Study:

• To elucidate the role of SlNAC90 in tomato's response to low-temperature stress.
• To identify the molecular mechanism regulating jasmonic acid (JA) biosynthesis under cold stress.

Main Methods:

• Gene expression analysis
• Protein-protein interaction studies
• Biochemical assays to measure JA and reactive oxygen species (ROS) levels.

Main Results:

• SlNAC90 directly regulates SlLOXC, promoting JA biosynthesis and enhancing low-temperature tolerance.
• SlLOXC overexpression increases JA and reduces ROS under cold stress; knockout has opposite effects.
• SlNAC90 interacts with SlNAC102, amplifying its regulatory function on SlLOXC.
• SlMYC2 transcriptionally activates both SlNAC90 and SlNAC102, forming a regulatory module.

Conclusions:

• The SlMYC2-SlNAC90-SlNAC102-SlLOXC module is central to JA biosynthesis regulation in tomato.
• This module plays a key role in mediating tomato's response to low-temperature stress via JA modulation.