Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hormones and Bone Tissue01:17

Hormones and Bone Tissue

3.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
3.7K
Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

4.5K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.5K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

3.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.9K
Skeleton and Calcium Homeostasis01:21

Skeleton and Calcium Homeostasis

5.7K
Calcium is not only the most abundant mineral in bone but also the most abundant mineral in the human body. Calcium ions are needed for bone mineralization, tooth health, heart rate regulation and strength of contraction, blood coagulation, the contraction of smooth and skeletal muscle cells, and the regulation of nerve impulse conduction. The average calcium level in the blood is about 10 mg/dL. When the body cannot maintain this level, a person will experience hypo or hypercalcemia.
5.7K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

6.0K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
6.0K
The Parathyroid Glands00:59

The Parathyroid Glands

4.3K
The two pairs of parathyroid glands embedded within the posterior surface of the thyroid gland are restricted by a dense capsule around them. These glands comprise two distinct cell populations—parathyroid oxyphil and parathyroid principal cells- pivotal in calcium homeostasis.
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The YTHDC1-m<sup>6</sup>A-GADD45B axis promotes chondrogenesis of hPDLSCs via suppressing senescence through p53/p21 signalling pathway.

International journal of oral science·2026
Same author

Expert consensus on orthodontic-associated alveolar ridge augmentation for adult patients.

International journal of oral science·2026
Same author

T2DM-Induced Gut Dysbiosis Exacerbates Periodontitis Through Intestinal Barrier Disruption and Redox Imbalance.

Journal of clinical periodontology·2026
Same author

Postmenopausal osteoporosis increases periodontal inflammation and the pathogenicity of the oral microbiota in a rat model.

Journal of oral microbiology·2025
Same author

Oxidative Stress and Gut Microbiota Interplay Exacerbates Periodontitis in Diabetic Mice.

Journal of clinical periodontology·2025
Same author

Enhanced Bone Repair using Callus Organoids Derived from Urine-Derived Stem Cells with Silk Fibroin.

Advanced healthcare materials·2025

Related Experiment Video

Updated: Jan 12, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

2.4K

p75NTR Promotes Circadian-Driven Mineralization During Tooth Development via CK2/PER2 Pathway.

Manzhu Zhao1,2,3,4, Hongyan Yuan1,2,3,4,5, Di Wang1,2,3,4

  • 1College of Stomatology, Chongqing Medical University, Chongqing, China.

Cell Proliferation
|November 4, 2025
PubMed
Summary

The p75 neurotrophin receptor (p75NTR) regulates daily tooth mineralization and development by synchronizing with circadian rhythms. Its absence disrupts these processes, impacting incisor eruption and growth.

Keywords:
p75 neurotrophin receptorcasein kinase 2circadian rhythmphosphorylationtooth development

More Related Videos

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

9.6K
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

5.0K

Related Experiment Videos

Last Updated: Jan 12, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

2.4K
Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
14:55

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Published on: June 24, 2018

9.6K
Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

5.0K

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Chronobiology

Background:

  • Circadian rhythms regulate physiological processes synchronized with light-dark cycles.
  • The role of circadian rhythm regulation in tooth development is not fully understood.
  • The p75 neurotrophin receptor (p75NTR) is involved in various biological processes.

Purpose of the Study:

  • To investigate the role of p75NTR in circadian rhythm regulation during tooth development.
  • To explore the mechanisms by which p75NTR influences daily mineralization and tooth morphogenesis.
  • To elucidate the interplay between circadian rhythm and dental development.

Main Methods:

  • Immunofluorescence and spatiotemporal analysis of rat dental germs.
  • Circadian rhythm tracking in p75NTR knockout mice (p75NTRExIII-/-).
  • Analysis of gene and protein expression, including clock genes, mineralization factors, and the CK2/PER2 pathway.

Main Results:

  • p75NTR expression oscillates in synchrony with clock genes and mineralization factors in developing rat teeth.
  • p75NTRExIII-/- mice show delayed incisor eruption, reduced mineralization, and altered clock gene expression.
  • p75NTR affects PER2 phosphorylation and nuclear accumulation via the CK2 pathway, impacting tooth development.

Conclusions:

  • p75NTR is crucial for regulating circadian-driven mineralization and tooth morphogenesis.
  • The p75NTR likely mediates its effects through the CK2/PER2 pathway.
  • This study provides critical insights into the connection between circadian rhythms and dental development.