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Aloperine Protects Against Cisplatin-Induced Injury in Kidney Cells Via Modulating PI3K/AKT/Nfκb-Mediated NLRP3

Mingning Qiu1,2,3, Shuai Zhang3, Jinglan Liang4

  • 1Key Laboratory of Research in Maternal and Child Medicine and Birth Defects, Guangdong Medical University, Foshan, 528300, Guangdong, China.

Protein and Peptide Letters
|November 4, 2025
PubMed
Abstract

Insights

Aloperine (ALO) protects kidney cells from cisplatin (CDDP) damage by inhibiting the PI3K/AKT/NFκB pathway and NLRP3 inflammasome. This study clarifies ALO

Area of Science:

  • Pharmacology
  • Toxicology
  • Cell Biology

Background:

  • Aloperine (ALO), an alkaloid from Sophora alopecuroides, exhibits anti-inflammatory properties.
  • The protective effects and mechanism of ALO against cisplatin (CDDP)-induced nephrotoxicity are not well understood.

Purpose of the Study:

  • To investigate the protective effects of ALO on CDDP-induced nephrotoxicity in vitro.
  • To elucidate the underlying molecular mechanisms, focusing on the PI3K/AKT/NFκB pathway and NLRP3 inflammasome.

Main Methods:

  • Cell viability, cytotoxicity (LDH), and apoptosis assays were used to assess kidney cell injury.
  • Western blotting and ELISA measured inflammatory factors and pathway activation.
  • Specific inhibitors (PI3K, AKT, NFκB) were employed to explore the mechanism of action.

Main Results:

  • ALO significantly reversed CDDP-induced decreases in cell viability, increases in cytotoxicity, and apoptosis.
  • ALO attenuated the activation of the PI3K/AKT/NFκB pathway and downregulated the NLRP3 inflammasome.
  • Inhibitor studies confirmed the involvement of the PI3K/AKT/NFκB pathway in ALO's protective effects.

Conclusions:

  • Aloperine demonstrates significant protective effects against CDDP-induced nephrotoxicity in vitro.
  • ALO exerts its protective effects by modulating the PI3K/AKT/NFκB signaling pathway and the NLRP3 inflammasome.
  • These findings highlight ALO as a potential therapeutic agent for preventing chemotherapy-induced kidney damage.
Keywords:
AloperineCDDP-induced injuryNLRP3 inflammasomePI3K/AKT/NFκBkidney cellssophora alopecuroides.

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