PtPdNi Trimetallic-Doped MIL-88 Hydrogel Accelerates Healing of Bacterial-Infected Diabetic Wounds
Yawen Peng1,2, Wenjuan Du3, Yating Cui1
1Medical School of Chinese PLA, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.
Purpose:
Treatment of bacterial-infected diabetic wounds is still challenging due to the susceptibility to bacterial infection and oxidative stress. Therefore, a novel multifunctional platform was established for accelerating the healing of bacterial-infected diabetic wounds.
Methods:
PtPdNi trimetallic-doped MIL-88 (PPNM) was fabricated and anchored to polyvinyl alcohol/sodium alginate (PVA/SA) matrix for constructing PPNM-containing hydrogel (PPNM-Gel). The basic properties of PPNM were characterized by TEM, SEM, XPS, FT-IR spectra, and UV-vis spectra. Adhesive, swelling, and degradation properties of PPNM-Gel were also analyzed. In vitro biocompatibility of PPNM-Gel was determined using L929 and HUVEC-SV40 cells. In vitro antimicrobial effects of PPNM-Gel were evaluated with E. coli and S. aureus. In vivo antibacterial and wound healing evaluation of PPNM-Gel was performed in the STZ-induced diabetic SD rats, which were established by intraperitoneal injection of STZ, followed by full-thickness skin wound preparation and bacterial infection. Histological and immunofluorescence staining were used to evaluate the status of epithelization, collagen deposition and inflammation response.
Results:
PPNM exhibited multienzyme-like activity including POD-like, OXD-like, CAT-like, SOD-like, and NAD-like activities. PPNM-Gel demonstrated excellent tissue adhesive activity, water absorption ability, biodegradability, and biocompatibility. In vitro testing, PPNM-Gel showed obvious antibacterial ability against E. coli and S. aureus in a PPNM dose-dependent approach, and its antibacterial ability against E. coli was stronger than that against S. aureus. In the STZ-induced diabetic SD rats, PPNM-Gel effectively accelerated wound remodeling by killing pathogenic bacteria, reducing inflammation, and promoting collagen deposition.
Conclusion:
PPNM-Gel significantly accelerated the healing of bacterial-infected diabetic wounds in the rat model. Therefore, PPNM-Gel showed great promise for the treatment of diseases affected by bacterial infections and high ROS levels.
