Blastocyst Structure-Inspired Core-Shell Stem Cell Microspheres with Hyperoside Delivery for Endometrial...

Area of Science:

• Biomaterials Science
• Regenerative Medicine
• Reproductive Biology

Background:

• Endometrial injury and intrauterine adhesions (IUA) significantly impact reproductive health.
• Current treatments for IUA have limitations in promoting complete endometrial regeneration.

Purpose of the Study:

• To develop a novel core-shell microsphere (HBM) for enhanced endometrial regeneration.
• To investigate the synergistic effects of bone marrow-derived mesenchymal stem cells (BMSCs) and hyperoside (HYP) delivered via HBM for IUA treatment.

Main Methods:

• Fabrication of HBM using microfluidic electrospray, featuring a magnetic nanoparticle-embedded shell (sodium alginate) and a stem cell-loaded core (carboxymethyl cellulose).
• In vitro assessment of HBM's effects on endothelial cell proliferation, angiogenesis, and inflammation.
• In vivo evaluation of HBM in a rat IUA model, assessing endometrial thickness, gland regeneration, collagen deposition, and inflammatory markers.

Main Results:

• HBM demonstrated successful spatiotemporal release of HYP and BMSCs, modulating macrophage polarization and promoting paracrine signaling.
• In vitro studies showed enhanced endothelial cell proliferation, angiogenesis, and inflammation inhibition.
• In vivo, HBM significantly restored endometrial structure, increased gland numbers, reduced fibrosis, and improved endometrial receptivity in the IUA model.

Conclusions:

• The developed HBM provides a promising synergistic strategy for endometrial regeneration by combining immunomodulation, stem cell therapy, and magnetic targeting.
• This approach offers a potential solution for preventing intrauterine adhesions and improving reproductive outcomes.