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Updated: Jul 5, 2026

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
Phage Display of Graspeptides via SrtA-Mediated Peptide Ligation and Cyclization
Jun-Hao Xue1, Hui-Min Wei1, Fan Wu1
1School of Life Sciences and Medical Engineering, Anhui University, Hefei 230601, P.R. China.
Abstract:
We report a chemoenzymatic strategy for generating graspeptide mimetics. This strategy exploits the compatibility of sortase with the ester group at the X position of the pentapeptide sequence (LPXTG). Six types of graspeptide mimetics, including monocyclic and bicyclic depsipeptides, were generated. Combined with phage display, we identified a bicyclic depsipeptide targeting TEAD4 with a KD value of 2.2 μM. This study demonstrates the potential of peptiligase-based system for constructing cyclic peptide libraries with unique topologies.
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