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Related Experiment Video

Updated: Jan 6, 2026

Preparation of Pooled Human Platelet Lysate pHPL as an Efficient Supplement for Animal Serum-Free Human Stem Cell Cultures
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Does platelet lysate switch cryopreserved human tissues on?

Sara Mirabet1, Néstor Villalba2, Vicente Mirabet3

  • 1BTE Lab, Fundación Hospital General Universitario, Valencia, Spain. saramitor@gmail.com.

Cell and Tissue Banking
|November 4, 2025
PubMed
Summary

Platelet lysate, derived from platelet-rich plasma, enhances cell proliferation, particularly when leukocytes are present. Its clinical use requires further study, as it showed no significant effect on thawed bone or vascular tissues.

Keywords:
Cell viabilityCryopreservationHuman tissuePlatelet lysatePlatelet-rich plasma

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Platelet lysate (PL) is a platelet-rich plasma derivative used in cell culture.
  • Clinical applications of PL are under-researched despite various preparation protocols.
  • Understanding PL's effects on tissue viability is crucial for clinical translation.

Purpose of the Study:

  • To compare the effects of different PL preparation protocols on cell proliferation.
  • To analyze the impact of PL on bone and vascular tissues.
  • To evaluate the long-term viability of stored bone and vascular tissues.

Main Methods:

  • Human fibroblast-like cells were used to assess PL preparation protocols.
  • Thawed bone and artery fragments were incubated with PL and cultured as explants.
  • Cell proliferation was quantified via cell counts and colony presence.
  • Viability of cryopreserved bone and vascular tissues was assessed over time.

Main Results:

  • Leukocyte presence in raw material correlated with increased cell proliferation in PL.
  • Artery fragments showed viable cells in all cultures; bone fragments showed 71.4% viability.
  • PL concentration did not significantly affect cell proliferation in thawed tissues.
  • Arteries stored in liquid nitrogen maintained viability for over 5 years.
  • Bone viability decreased after 3 months when stored at -80°C.

Conclusions:

  • Lymphocytes contribute bioactive substances that promote cell growth.
  • Platelet lysate incubation did not significantly activate cells in thawed tissues.
  • Long-term cryopreservation of arteries is feasible, while bone viability is limited.
  • Further research is needed to optimize PL for clinical applications.