De novo transcriptome analysis of Perna perna L. (Bivalve) with functional and metabolic pathway analysis

Mussels, particularly Indian brown mussels (Perna perna L.), have great ecological and economic value due to their restricted distribution along the Indian coastline. They are ideal model organisms for various study domains such as climate change adaptability, biomonitoring, biomaterials, bioadhesion, biofouling, and antifouling. However, there is a noticeable scarcity of genetic information about this species. There is no previous transcriptomic study of the Indian brown mussel. This study used a de novo transcriptomic technique to generate a mussel foot-specific transcriptome for P. perna using 31.72 million high-quality Illumina paired-end reads. A total of 33,567 unigenes were generated, and 18,951 coding sequences (CDS) were predicted. We identified several gene families and key functional genes, including Cytochrome P450 (CYP2, CYP1, CYP3, CYP4), Heat-shock proteins (HSP-70, HSP-90), Superoxide dismutase (SOD), Catalase (CAT), Glutathione-S transferase (GST), Toll-like receptors (TLR-13, TLR-8, TLR-7), Aquaporins (AQP-4, AQP-8), growth factor (IGF), Glutathione peroxidase, Acetylcholinesterase (AchE), Hypoxia-inducible factor-2 and proteins and enzymes involved in bio-adhesion. Our current study serves as a platform for future functional and comparative transcriptomics studies.