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Related Experiment Video

Updated: Jan 12, 2026

Selective Harvesting of Marginating-hepatic Leukocytes

Selective Harvesting of Marginating-hepatic Leukocytes

Published on: July 21, 2016

Discovering Heterogeneous Leukocytes Subsets Associated With Alcoholic Steatohepatitis by scRNAseq Analysis.

Haribalan Perumalsamy^1,2,3, Sehee Park³, Ji Eun Kim⁴

¹Research Institute for Convergence of Basic Science Hanyang University Seongdong-gu Seoul Republic of Korea.

|November 5, 2025

Summary

This summary is machine-generated.

This study reveals how immune cells change in alcoholic steatohepatitis (ASH). Alcohol exposure alters B cells and neutrophils, impacting liver fibrosis and identifying potential biomarkers for alcoholic liver disease (ALD).

Keywords:

B cell alcoholic liver disease alcoholic steatohepatitis circulating immune cells neutrophils scRNAseq tSNEs

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Last Updated: Jan 12, 2026

Selective Harvesting of Marginating-hepatic Leukocytes

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Published on: July 21, 2016

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

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Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Mass Cytometry Analysis of Systemic and Local Immune Responses in Hepatocellular Carcinoma

Published on: April 25, 2025

Area of Science:

Immunology
Hepatology
Genomics

Background:

Alcoholic steatohepatitis (ASH) pathogenesis involves complex immune responses.
Single-cell analysis is crucial for understanding immune cell heterogeneity in liver disease.

Purpose of the Study:

To precisely identify immune cell type responses in alcoholic liver disease (ALD) at the single-cell level.
To explore the role of specific immune cell populations in alcohol-induced liver fibrosis.

Main Methods:

High-dimensional single-cell RNA sequencing was performed on mouse models of ALD.
t-distributed stochastic neighbor embedding (t-SNE) was used for dimensionality reduction and visualization.
Automated cell annotation using singleR identified cell types and differentially expressed genes.

Main Results:

A decline in B cell populations and altered gene expression indicated an innate proinflammatory response linked to fibrosis.
Neutrophil deficiency was observed in alcohol-induced ASH.
Increased eosinophils suggested functional heterogeneity within granulocyte subsets and potential complications in liver fibrosis.

Conclusions:

Findings highlight the significant impact of alcohol exposure on immune cell populations, particularly B cells and neutrophils.
The study identifies potential biomarker cell types for ALD that are reduced by alcohol consumption.
This research enhances understanding of circulating immune leukocytes contributing to alcohol-induced liver fibrosis.