Related Experiment Video
Updated: Jan 12, 2026
Author Spotlight: Insights into Cardiometabolic Diseases with Subcutaneous Adipose Tissue Microvasculature Studies
Published on: April 5, 2024
Comparative evaluation of hypoxia preconditioned serum (HPS) and platelet-rich plasma (PRP) on preadipocyte survival
Jun Jiang1, Michael Martin1, Lynn Röper1
1Experimental Plastic Surgery, Clinic for Plastic, Reconstructive and Hand Surgery, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany.
Introduction:
Lipofilling is a widely used technique in plastic and reconstructive surgery, but its long-term success is often limited by unpredictable fat graft resorption. Optimizing the adipogenic environment through bioactive factors may enhance graft survival and volume retention. This study investigates the adipogenic potential of Hypoxia Preconditioned Serum (HPS) and Platelet-rich Plasma (PRP), in comparison to normal serum (NS).
Methods:
Cytokine profiles of HPS, PRP, and NS from 10 donors were analyzed. Human preadipocytes (n = 3) were cultured with low (10%) and high (40%) concentrations of these secretomes. Proliferation, cytotoxicity (LDH assay), lipid droplet formation (Oil Red O staining), and gene expression (qPCR) of adipogenic markers (PPARgamma, C/EBPalpha, FABP4, Adiponectin, LPL) were assessed after 2 and 4 days.
Results:
HPS contained significantly higher levels of Adiponectin, IGF-1, bFGF, VEGF-A, and PDGF-BB compared with PRP and NS, while Leptin was lower in HPS and PRP than in NS. All conditions increased proliferation on day 4, with the highest cell counts in NS-40%. No treatment-related cytotoxicity was observed. HPS-40% induced the strongest adipogenic differentiation, evidenced by increased lipid droplet formation and upregulation of all measured adipogenic genes by day 4.
Conclusion:
These findings suggest that HPS enhance the proliferation, survival, and differentiation of preadipocytes. Validation in in vivo models and clinical studies will be necessary to confirm its potential efficacy in enhancing graft survival and volume retention.
