Caballeronia eucalypticola sp. nov., isolated from the xylem tissues of eucalyptus

A Gram-stain-negative, aerobic, ovoid or rod-shaped, non-spore-forming, motile with peritrichous flagella bacterium, designated strain N3-1-1-11^T, was isolated from the xylem tissues of Eucalyptus grandis × Eucalyptus urophylla in Qinzhou, Guangxi Province, China. Optimal growth occurred at 28 °C, at pH 6.0 and in the presence of 0-1% (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that N3-1-1-11^T was clustered with species of the genus Caballeronia and formed a distinct sublineage, showing the highest similarities to Caballeronia mineralivorans PML1(12)^T (98.48%), Caballeronia udeis LMG 27134^T (97.99%) and Caballeronia sordidicola LMG 22029^T (97.86%). The predominant ubiquinone is Q-8. The major fatty acids are C_16 : 0, C_17 : 1  ω5c, summed feature 3 (C_16 : 1  ω6c and/or C_16 : 1  ω7c) and C_18 : 1  ω7c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 59.33 mol%. The phylogenetic analysis and physiological and biochemical data showed that strain N3-1-1-11^T should be classified as representing a novel species in the genus Caballeronia, for which the name Caballeronia eucalypticola sp. nov. is proposed. The type strain of C. eucalypticola is N3-1-1-11^T (=CFCC 15711^T=CGMCC 1.65219^T=KCTC 8740^T).