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Related Experiment Video

Updated: Jan 12, 2026

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

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Protocol to quantify protein phosphorylation in mouse extended pluripotent stem cell-derived blastoids.

Tianxia Xiao1, Yuxin Luo2, Yang Yu3

  • 1Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology and Key Laboratory of Assisted Reproduction, Ministry of Education, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.

STAR Protocols
|November 5, 2025
PubMed
Summary

This study details a proteomics protocol for quantifying protein phosphorylation in mouse extended pluripotent stem cell (EPSC)-derived blastoids. The standardized workflow ensures reproducible phospho-proteome analysis for stem cell research.

Keywords:
Developmental biologyProteomicsStem CellsSystems biology

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Last Updated: Jan 12, 2026

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Area of Science:

  • Proteomics
  • Stem Cell Biology
  • Post-translational Modifications

Background:

  • Protein phosphorylation regulates critical cellular functions.
  • Understanding phosphorylation in early embryonic development is crucial.
  • Extended pluripotent stem cells (EPSC) and blastoids model early development.

Purpose of the Study:

  • To present a standardized proteomics protocol for quantifying protein phosphorylation in mouse EPSC-derived blastoids.
  • To establish a reproducible workflow for phosphoproteome analysis in this model system.

Main Methods:

  • Blastoid culture from Mus musculus.
  • Sample preparation and phosphopeptide enrichment.
  • High-resolution liquid chromatography-mass spectrometry (LC-MS) data acquisition and analysis.

Main Results:

  • A comprehensive workflow for analyzing the phosphoproteome of mouse blastoids was established.
  • The protocol enables quantitative analysis of protein phosphorylation patterns.

Conclusions:

  • This protocol provides a robust framework for reproducible phospho-proteome analysis in mouse blastoids.
  • Facilitates deeper understanding of phosphorylation-driven regulatory networks in early development.