Biofunctionalization of selenium nanoparticles using a Sonneratia caseolaris (L.) leaves polysaccharide: Statistical...

Area of Science:

• Materials Science
• Nanotechnology
• Biotechnology

Background:

• Selenium nanoparticles (SeNPs) are gaining attention for their therapeutic potential.
• Stabilizing SeNPs with biocompatible materials is crucial for their application.
• Mangrove polysaccharides offer unique properties for nanoparticle synthesis.

Purpose of the Study:

• To optimize the synthesis of selenium nanoparticles (SeNPs) using Sonneratia caseolaris mangrove polysaccharide (SCLP).
• To characterize the physicochemical properties, stability, and biological activities of SCLP-SeNPs.
• To evaluate the potential of SCLP-SeNPs as antioxidant and antibiofilm agents in food applications.

Main Methods:

• Central Composite Design (CCD) for synthesis optimization.
• Characterization using XRD, Raman spectroscopy, and thermal analysis.
• In vitro antimicrobial, antibiofilm, and antioxidant assays.
• In vitro and in vivo toxicity assessments.

Main Results:

• Optimized synthesis yielded monodisperse spherical SCLP-SeNPs (approx. 81 nm).
• SCLP provided excellent storage stability (>2 months at 4°C).
• SCLP-SeNPs demonstrated enhanced antimicrobial, antibiofilm, and radical scavenging activities.
• Reduced toxicity and excellent biocompatibility were observed for SCLP-SeNPs.

Conclusions:

• SCLP is an effective stabilizer for SeNP synthesis.
• SCLP-SeNPs exhibit superior biological activities and biocompatibility compared to bare SeNPs.
• SCLP-SeNPs show significant potential as antioxidant and antibiofilm agents for food applications.