Related Experiment Video
Updated: Jan 12, 2026
Analyses of Proteinuria, Renal Infiltration of Leukocytes, and Renal Deposition of Proteins in Lupus-prone MRL/lpr Mice
Published on: June 8, 2022
TRIM8-associated non-coding RNA panel as a biomarker for Lupus nephritis activity
Mostafa Abdelnasier Abd Elgawad1, Howayda Abdelhamid El Shinnawy1, Sanaa Eissa2
1Internal Medicine and Nephrology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, 11566, Egypt.
Background:
Lupus nephritis (LN) represents a major complication in systemic lupus erythematosus (SLE). The objective of this study was to evaluate the TRIM8 gene and its associated non-coding RNAs (lnc-SSBP2-1:1 and hsa-miR-126-5p) as potential non-invasive biomarkers for LN activity.
Methods:
Bioinformatics analyses were initially employed to identify candidate mRNA and associated non-coding RNAs (ncRNAs) implicated in LN. Expression profiles of TRIM8lnc-SSBP2-1:1and hsa-miR-126-5p were validated in blood samples from 40 active LN, 30 inactive LN patients, and 20 healthy individuals via real-time PCR.
Results:
TRIM8 mRNA and lnc-SSBP2-1:1 lncRNA levels were notably upregulated in active LN (p < 0.001), while hsa-miR-126-5p was reduced (p < 0.001). SLEDAI-2K scores correlated positively with TRIM8 mRNA and lnc-SSBP2-1:1, and negatively with hsa-miR-126-5p.
Conclusions:
This study highlights TRIM8-associated ncRNA regulatory network as promising biomarkers in LN activωity, with potential clinical impact.
More Related Videos
11:35Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
Published on: August 21, 2016
09:16Supervised Machine Learning for Semi-Quantification of Extracellular DNA in Glomerulonephritis
Published on: June 18, 2020
