Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hand hygiene

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...

Cleaning, Sterilization, and Disinfection

Cleaning, Sterilization, and Disinfection

Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...

Methods of Sterilization I: Physical Methods

Methods of Sterilization I: Physical Methods

As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A microstructurally motivated framework to study autoregulation in the coronary circulation.

The Journal of physiology·2026

Same author

Functional consequences of diminished myocardial oxygen delivery per beat in experimental heart failure.

Basic research in cardiology·2026

Same author

Systems Analysis of Carboxylate Transport and Oxidation Pathways in Cardiac Mitochondria.

bioRxiv : the preprint server for biology·2026

Same author

Soluble Thrombomodulin Links Viremia and Mortality During COVID-19: Results From the ACTIV-4a Trial.

Journal of the American Heart Association·2026

Same author

Analyzing the role of ATP-dependent potassium channels in the regulation of coronary metabolic vasodilation during exercise.

Basic research in cardiology·2026

Same author

A stromal PAI1-tPA axis orchestrates immunosuppression in pancreatic cancer.

Science advances·2026

Search research articles

Related Experiment Video

Updated: Jun 19, 2026

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Immersion-Based Clearing and Autofluorescence Quenching in Myocardial Tissue.

Victoria E Sturgess¹, Nadia K Korovesis¹, Domingo E Uceda²

¹Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA.

Microcirculation (New York, N.Y. : 1994)

|November 6, 2025

Summary

This study optimized an immersion-based protocol for 3D microvascular imaging in myocardial tissues using tomato lectin and CUBIC clearing. The method enhances practical visualization of coronary microvasculature in larger animal models.

Keywords:

CUBIC confocal microscopy delipidation imaging depth myocardial microvasculature

More Related Videos

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Published on: May 31, 2017

Simultaneous 3D Analysis of Cardiac Damage and Immune Response in Reperfused Acute Myocardial Infarction Using Light Sheet Fluorescence Microscopy

Simultaneous 3D Analysis of Cardiac Damage and Immune Response in Reperfused Acute Myocardial Infarction Using Light Sheet Fluorescence Microscopy

Published on: September 26, 2025

Related Experiment Videos

Last Updated: Jun 19, 2026

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Imaging Cleared Embryonic and Postnatal Hearts at Single-cell Resolution

Published on: October 7, 2016

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

Published on: May 31, 2017

Simultaneous 3D Analysis of Cardiac Damage and Immune Response in Reperfused Acute Myocardial Infarction Using Light Sheet Fluorescence Microscopy

Simultaneous 3D Analysis of Cardiac Damage and Immune Response in Reperfused Acute Myocardial Infarction Using Light Sheet Fluorescence Microscopy

Published on: September 26, 2025

Area of Science:

Biomedical Engineering
Optical Microscopy
Histology

Background:

3D visualization of microvascular networks is advancing with new optical microscopy and tissue preparation techniques.
Tissue clearing improves imaging depth by enhancing light penetration.
Immersion-based methods offer practical advantages over perfusion methods for larger tissue samples.

Purpose of the Study:

To present an optimized immersion-based protocol for microvascular labeling and tissue clearing of myocardial tissues.
To demonstrate the efficacy of tomato lectin and CUBIC clearing for deep tissue imaging.
To assess the impact of optimized delipidation and quenching stages on image quality.

Main Methods:

Developed an immersion protocol using tomato lectin and CUBIC clearing for rat and pig myocardial tissues.
Optimized delipidation and quenching steps for enhanced imaging.
Evaluated image quality using signal-to-noise ratio (SNR) and average z-slice intensities.

Main Results:

Optimal image quality was achieved with a 24-hour incubation in CUBIC Reagent I.
Certain quenching agents (TrueBlack, Sudan Black B) showed a trend towards reduced imaging depth.
Rat myocardial tissues exhibited higher SNRs compared to pig tissues.

Conclusions:

The developed protocol provides a robust method for optimizing immersion-based myocardial tissue clearing.
This technique facilitates detailed 3D imaging of coronary microvasculature.
Future research will utilize this protocol to compare microvascular anatomy in different disease states.