Multifunctional Edged-Satellite AuAg Nanoparticles-Based Integration Platform for Screening, Validation, and...

Multifunctional materials converge various physical and chemical properties, while often being hampered by complicated synthesis procedures and inconsistent performance. A straightforward strategy is presented for synthesizing robust multifunctional plasmonic nanoparticles (NPs), AuAgNPs with edged satellites (ES-AuAgNPs). The ES-AuAgNPs possess various characteristics of i) alloyed Ag/Au with a 7:3 ratio, ii) numerous satellited NPs with narrow nanogaps on face-to-face edges, and iii) a well-accessible surface deposited with antimicrobial components. These properties endow ES-AuAgNPs with improved peroxidase (POD)-like catalytic, surface-enhanced Raman scattering (SERS), and antibacterial activities. By utilizing the triple-performance of ES-AuAgNPs and a modified specific aptamer probe, a portable, modular, and centimeter-sized platform is designed for simultaneously screening, validating, and eliminating pandemic marine Vibrio bacteria: parahaemolyticus, vulnificus, and alginolyticus. Wherein, POD-like catalytic-based colorimetric capacity can rapidly screen the presence of these bacteria through visible color change, and SERS-based detection validates bacterial identity with multiplexing capability. Meanwhile, the inherent antibacterial properties of ES-AuAgNPs ensure the effective elimination of detected bacteria. Remarkable performance is achieved in rapid screening (2 min), sensitive validation (limit of detection, ≈50 CFU per mL), and efficient antimicrobility (45 min, 100%). The ES-AuAgNPs constructed platform is believed to improve aquaculture management by seamlessly analyzing and eliminating bacteria.