Effects of Different Doses of Nanoparticle Fullerenol C60 on Wound Healing in Rats

The aim of current study was to investigate the effects of three different doses of nanoparticle fullerenol C60 (F-C60), which has an antioxidant and anti-inflammatory effects, on wound healing in rats with an experimental excisional wound model. Rats were divided into four groups (n = 7). F-C60 was administered at dose of 0.2 mg/kg intraperitoneal (i.p.) to group lower-dose F-C60, 1 mg/kg i.p. to group middle-dose F-C60, and 5 mg/kg i.p. to group higher-dose F-C60 30 min before from anesthesia. The group control received i.p. physiological saline. A wound was created via excisional model under anesthesia and rats were sacrificed 3 days after wound formation. Wound healing percentages were found by measuring wound diameters. Interleukins (IL-1β, IL-6), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), glutathione peroxidase (GPx), myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT), reactive oxygen species (ROS) were assessed. Histopathological analysis was performed. Kruskal Wallis and one-way ANOVA with post-hoc Tukey tests were used for statistical analysis (p < 0.05). IL-1β, IL-6, MPO, and MDA levels were statistically lower in group higher-dose F-C60 compared to the group control. Wound healing percentages of the groups were similar. There were no statistically significant differences between the groups in terms of TNF-α, ROS, SOD, CAT, and GPx levels, but the levels of antioxidant enzymes were lower in the group higher-dose F-C60. There were no statistically significant differences between the groups in terms of histopathological parameters. F-C60 promoted wound healing by reducing the level of pro-inflammatory cytokines such as IL-1β, IL-6 and alleviating inflammation. The current study was terminated on the 3rd postoperative day and this is may be the reason the unchanged results of some parameters such as wound healing percentages and histopathological parameters. F-C60 also demonstrated ameliorative effects on wound by reducing oxidative stress, lipid peroxidation, and by preventing the production of antioxidant enzymes. As a result, F-C60 may be a potential agent with these positive effects on wound healing.