Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
2.1K
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

6.4K
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
6.4K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

8.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
8.6K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

2.0K
2.0K
Protein Complex Assembly02:41

Protein Complex Assembly

16.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Walking the Tightrope: Balancing Opposing Cooperativities as an Operating Principle in Dynein Assembly.

bioRxiv : the preprint server for biology·2026
Same author

Multiple data set Bayesian analysis synergistically boosts ITC parameter precision.

Biophysical journal·2026
Same author

Cooperative multivalency converts disorder into rods, resolving a paradox in cellular architecture.

bioRxiv : the preprint server for biology·2026
Same author

Improving parameter inference by resolving Bayesian prior ambiguity via multi-dataset analysis: Application to isothermal titration calorimetry.

bioRxiv : the preprint server for biology·2025
Same author

Successful prediction of LC8 binding to intrinsically disordered proteins sheds light on AlphaFold's black box.

Frontiers in molecular biosciences·2025
Same author

LC8 enhances 53BP1 foci through heterogeneous bridging of 53BP1 oligomers.

eLife·2025
Same journal

PDC1 deficiency results in 2-deoxyglucose sensitivity through inhibition of Pdc2 activity in yeast.

The FEBS journal·2026
Same journal

Epigenetic regulation of the hepcidin gene expression in hepatoma cells.

The FEBS journal·2026
Same journal

Loss of Ambp ameliorates steatosis progression by activating PPARα signaling in zebrafish.

The FEBS journal·2026
Same journal

Varying susceptibility of subpopulations along the epithelial-mesenchymal spectrum to undergo EMT.

The FEBS journal·2026
Same journal

ALOX15 links lipid metabolism to receptor trafficking in platelet activation.

The FEBS journal·2026
Same journal

A two-component ortholog of the Niemann-Pick C 1 protein is essential for normal growth and sterol trafficking and signaling in Tetrahymena thermophila.

The FEBS journal·2026
See all related articles

Related Experiment Video

Updated: Jan 12, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.5K

Multifaceted multivalency with a hub protein.

Douglas R Walker1, Elisar J Barbar1

  • 1Department of Biochemistry & Biophysics, Oregon State University, Corvallis, USA.

The FEBS Journal
|November 6, 2025
PubMed
Summary
This summary is machine-generated.

LC8 protein interactions are better understood by studying its multivalency, focusing on registration, binding sites, and flexibility. This research helps predict how LC8 partners assemble and the functional outcomes.

Keywords:
complex regulationhub proteinintrinsically disordered proteinslinker lengthmultivalency

More Related Videos

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP
06:45

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP

Published on: June 15, 2018

7.9K
Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

31.8K

Related Experiment Videos

Last Updated: Jan 12, 2026

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.5K
Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP
06:45

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification BiCAP

Published on: June 15, 2018

7.9K
Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions
14:04

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

31.8K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • LC8 protein is known for its role in various cellular processes.
  • Understanding LC8 multivalency is crucial for deciphering its complex interactions.
  • GKAP ( ankyrin repeat and SOCS box-containing protein 1) is a key partner in these interactions.

Purpose of the Study:

  • To elucidate the mechanisms governing polybivalent complexation behavior of LC8.
  • To investigate the impact of registration control, binding motifs, linker lengths, and flexibility on LC8 interactions.
  • To establish a predictive framework for LC8-partner complex formation and function.

Main Methods:

  • Analysis of LC8-GKAP interactions.
  • Computational modeling to assess linker flexibility and binding site registration.
  • Sequence-based analysis of LC8 binding partners.

Main Results:

  • Identified key features (registration, motifs, linkers, flexibility) governing LC8 multivalency.
  • Demonstrated the ability to predict assembly characteristics (homogeneous/heterogeneous) based on sequence.
  • Showed prediction of binding strength and functional consequences is achievable.

Conclusions:

  • LC8 multivalency is a complex phenomenon governed by specific molecular features.
  • Sequence analysis provides a powerful tool for predicting LC8-partner complex behavior.
  • This work advances the understanding of how protein sequence dictates complex functional outcomes.