Related Experiment Video
Updated: Jan 12, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
Published on: January 18, 2017
ZNF582 Inhibits Head and Neck Squamous Cell Carcinoma Progression and Stemness via MAPK and PI3K/AKT Signaling
Yaqing Mao1,2, Shuo Liu1,2, Zhen Qin1,2
1Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, Beijing, People's Republic of China.
Background:
Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy characterized by high metastatic potential and poor prognosis. Previous studies have focused on the correlation between hypermethylated ZNF582 and HNSCC prognosis, particularly in diagnostic contexts, but the underlying molecular mechanisms remain poorly understood. This study explored the biological function and underlying mechanisms of ZNF582 in HNSCC.
Methods:
ZNF582 expression was analyzed in HNSCC and non-tumor tissues. Functional assays, including colony formation, wound healing, Transwell, TUNEL assays, and xenograft models, were performed in HNSCC cell lines with or without ZNF582 overexpression. To assess cancer stemness, fluorescence-activated cell sorting, tumorsphere formation assay, extreme limiting dilution assay, and an orthotopic model were used. High-throughput RNA sequencing was performed to identify the altered signaling pathways.
Results:
Bioinformatics analysis revealed that low ZNF582 expression in HNSCC correlated with poor prognosis. Overexpression of ZNF582 suppressed HNSCC proliferation, migration, and invasion, while promoting apoptosis both in vitro and in vivo. ZNF582 also reduced cancer stem cell activity and metastasis in HNSCC. Mechanically, ZNF582 overexpression inhibited the MAPK and PI3K/AKT signaling pathways.
Conclusion:
Our findings suggested that ZNF582 overexpression inhibits HNSCC progression via the MAPK and PI3K/AKT signaling pathways, highlighting ZNF582 as a potential therapeutic target gene for HNSCC treatment.
Related Concept Videos
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The...
MAPK Signaling Cascades
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
mTOR Signaling and Cancer Progression
The mTOR pathway or the...
PI3K/mTOR/AKT Signaling Pathway
Inhibition of Cdk Activity

