Related Experiment Video
Updated: Jan 12, 2026

Scanning Electron Microscopy of Macerated Tissue to Visualize the Extracellular Matrix
Published on: June 14, 2016
CXCL1 Promotes Fibrotic Remodeling in Atrial Fibrillation via Activation of TXNDC5 and Endoplasmic Reticulum Stress
Ran Yin1, Cheng-Long Wu1, Si-Yang Yu1
1Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, China.
Background:
Atrial fibrosis is a key structural substrate in atrial fibrillation (AF). This work was conducted to investigate the profibrotic effects of chemokine C-X-C motif Ligand 1 (CXCL1) and elucidate the actions of endoplasmic reticulum stress (ERS) and ER-resident protein thioredoxin domain-containing Protein 5 (TXNDC5) in this process.
Methods:
Serum CXCL1 concentrations were measured in patients with AF and healthy controls. The effects of CXCL1 on atrial fibrosis were evaluated using ex vivo rat atrial tissue culture. Additionally, the influence of CXCL1 on collagen synthesis, ERS activation, and TXNDC5 expression was assessed in primary rat cardiac fibroblasts. Pharmacological inhibition of ERS and gene silencing of TXNDC5 were employed to decipher underlying mechanisms.
Results:
CXCL1 levels were elevated in patients with AF compared to controls. In ex vivo rat atrial tissue, CXCL1 treatment induced marked fibrosis and upregulated the expression of ERS markers GRP78 and ATF6, as well as TXNDC5. In cardiac fibroblasts, CXCL1 promoted the secretion of Collagen I, Collagen III, and TGF-β1. Notably, both ERS inhibition and TXNDC5 knockdown effectively attenuated CXCL1-induced fibroblast activation and extracellular matrix protein expression.
Conclusion:
CXCL1 promotes atrial fibrosis through the activation of ERS and upregulation of TXNDC5, potentially contributing to atrial remodeling and the pathogenesis of AF. Targeting the CXCL1-ERS-TXNDC5 axis may offer a novel therapeutic approach for preventing and treating AF-related atrial fibrosis.
Related Concept Videos
Cell-matrix's Response to Mechanical Forces
Anchoring junctions mechanically attach a cell to the...
Intracellular Signaling Affects Focal Adhesions
Some...
Clot Retraction and Fibrinolysis
TGF - β Signaling Pathway
Rheumatic Heart Disease I: Introduction

