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Related Experiment Video

Updated: Jul 18, 2026

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood
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Sequential trench well based microfluidic platform to isolate bacteria from whole blood with large volume processing.

Cheonggyu Lee1, Gi Yoon Lee2, Hyerin Joo3

  • 1Mechanical Engineering, Ajou University, 206, World cup-ro, Yeongtong-gu, Suwon-si, Gyeonggi-do, Republic of Korea. hajh@ajou.ac.kr.

Lab on a Chip
|November 6, 2025
PubMed
Summary

This study introduces a microfluidic device for efficient bacteria separation from blood, achieving over 99.99% red blood cell removal and up to 78% bacterial recovery. This method significantly speeds up antimicrobial susceptibility testing for infection diagnostics.

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Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Infectious Disease Diagnostics

Background:

  • Effective bacterial separation from blood is vital for timely infection diagnosis.
  • Low bacterial loads and high blood cell counts pose significant challenges to current separation methods.

Purpose of the Study:

  • To develop and validate a microfluidic system for efficient bacteria isolation from whole blood.
  • To assess the system's performance in terms of red blood cell removal and bacterial recovery rates.
  • To evaluate the impact of this separation technique on accelerating antimicrobial susceptibility testing.

Main Methods:

  • A microfluidic device with a sequential trench well structure was designed.
  • Sedimentation-based separation principles were employed for bacterial isolation.
  • The system's efficiency was evaluated using red blood cell removal and bacterial recovery metrics.
  • The isolated bacteria were used for image-based antimicrobial susceptibility testing (AST).

Main Results:

  • The microfluidic system achieved over 99.99% red blood cell (RBC) removal.
  • Bacterial recovery rates reached up to nearly 78%.
  • The integrated approach reduced the conventional AST procedure time by up to 42.18 hours.

Conclusions:

  • The developed microfluidic platform offers a simple, effective method for isolating bacteria from blood.
  • This technology has significant potential for improving the speed and efficiency of infection diagnostics.
  • The approach streamlines microbiological analysis and antimicrobial susceptibility testing processes.