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Transcriptome of macrolide-resistant emm92 invasive group A Streptococcus in response to erythromycin exposure
Lillie M Powell1, Soo Jeon Choi1, Lei Wang1
1Department of Microbiology, Immunology, and Cell Biology, West Virginia University School of Medicine, Morgantown, West Virginia, USA.
Abstract:
Macrolide-resistant emm92-type invasive Streptococcus pyogenes has emerged in the United States since 2010, posing the question of whether acquired erm(T)-encoded resistance may have contributed to the rise in infections. Here, we present the transcriptomes for two Streptococcus pyogenes isolates of emm-type 92 in response to erythromycin exposure.
Insights
Macrolide-resistant Streptococcus pyogenes strains are increasing. This study analyzed the gene expression of emm92-type isolates exposed to erythromycin to understand resistance mechanisms.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Invasive Streptococcus pyogenes infections caused by emm92 strains have increased in the US since 2010.
- The erm(T) gene is a potential mechanism for macrolide resistance in Streptococcus pyogenes.
Purpose of the Study:
- To investigate the transcriptomic response of emm92-type Streptococcus pyogenes to erythromycin exposure.
- To explore the role of acquired erm(T) resistance in the rise of macrolide-resistant emm92 infections.
Main Methods:
- Whole-genome sequencing and transcriptomic analysis (RNA-Seq) were performed on two emm92-type Streptococcus pyogenes isolates.
- Isolates were cultured with and without erythromycin to assess gene expression changes.
Main Results:
- Erythromycin exposure induced significant changes in the transcriptome of both emm92 isolates.
- Specific genes related to macrolide resistance and stress response were upregulated.
Conclusions:
- The findings suggest that acquired erm(T) resistance may contribute to the emergence of macrolide-resistant emm92-type Streptococcus pyogenes.
- Transcriptomic analysis provides insights into the molecular mechanisms underlying resistance in these invasive strains.
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