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Related Experiment Video

Updated: Jan 12, 2026

Author Spotlight: Enhancing Graft Viability Assessment Through Quantitative Metrics and Innovative Reservoir Systems

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Published on: August 2, 2024

Advancing Assessment Methods for Human Cardiac Grafts During Normothermic Machine Perfusion.

Emmanuella O Ajenu¹, Manuela Lopera Higuita¹, Maya Bolger-Chen¹

¹Department of Surgery, Center for Engineering in Medicine and Surgery, Harvard Medical School and Massachusetts General Hospital, Shriners Children's Boston, Boston, MA.

Transplantation

|November 6, 2025

Summary

This summary is machine-generated.

New assessment methods using normothermic machine perfusion (NMP) can improve donor heart selection for transplantation. These techniques evaluate mitochondrial function, heart performance, and inflammatory markers, potentially increasing the use of available donor hearts.

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Last Updated: Jan 12, 2026

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Published on: January 12, 2019

Rat Model of Normothermic Ex-Situ Perfused Heterotopic Heart Transplantation

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Published on: April 21, 2023

Area of Science:

Cardiovascular Surgery
Transplantation Medicine
Biomedical Engineering

Background:

Heart failure impacts over 23 million globally, with transplantation limited by donor heart scarcity and strict selection criteria.
Normothermic machine perfusion (NMP) shows potential for improving graft preservation and assessment, but its role in predicting transplantability needs further exploration.
Current limitations in donor heart utilization underscore the need for advanced evaluation techniques.

Purpose of the Study:

To investigate three novel assessment methods for human donor hearts during normothermic machine perfusion (NMP).
To enhance the evaluation of graft viability and predict post-transplant outcomes.
To explore the potential of NMP in optimizing donor heart selection and utilization.

Main Methods:

Utilized resonance Raman spectroscopy to assess mitochondrial redox state, a marker of metabolic function during ex vivo preservation.
Quantified left ventricular performance using intraventricular balloons to gauge graft viability.
Analyzed perfusate for inflammatory markers and endothelial activation to predict transplant outcomes.

Main Results:

Successfully applied the three assessment methods to human donor hearts declined for transplantation.
Hearts were preserved using static cold storage and subsequently evaluated with NMP in Langendorff mode.
Demonstrated the feasibility of integrating these parameters into clinical perfusion workflows.

Conclusions:

The assessed parameters show potential for improving heart transplant outcomes through enhanced graft selection.
These methods can help optimize the utilization of donor hearts, addressing scarcity issues.
Further validation across diverse preservation techniques and clinical applicability studies are warranted.