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Updated: Jan 12, 2026

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney
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Deep-supercooling Preservation for Rat Kidneys.

Yunkai Li1,2, Zhijie Li1,2, Lanlan Peng1,2

  • 1The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Xi'an Jiaotong University, Xi'an, China.

Transplantation
|November 6, 2025
PubMed
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Deep-supercooling (DSC) preservation at -10°C extends kidney viability fourfold compared to static cold storage (SCS). This novel method improves graft quality and addresses organ shortages for transplantation.

Area of Science:

  • Biomedical Engineering
  • Organ Transplantation
  • Cryobiology

Background:

  • Critical shortage of transplantable organs necessitates improved preservation methods.
  • Static cold storage (SCS) limits kidney preservation to 24 hours at 4°C.
  • Suboptimal efficacy of SCS drives the need for advanced preservation technologies.

Purpose of the Study:

  • To pioneer deep-supercooling (DSC) for ice-free rat kidney preservation at -10°C.
  • To systematically compare DSC efficacy against conventional SCS.
  • To establish the maximum viable preservation window for DSC kidney storage.

Main Methods:

  • Rat kidneys were preserved using SCS (4°C) or DSC (-10°C) for 24, 96, or 168 hours.
  • Normothermic reperfusion was performed on subset groups.

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  • Comprehensive biomarker profiling assessed functional, metabolic, and structural integrity.

    • Main Results:

    • DSC maintained stable supercooled preservation at -10°C.
    • 24-hour DSC storage showed superior functional recovery, enhanced glomerular filtration, optimized energy homeostasis, and attenuated ischemic injury compared to SCS.
    • 96-hour DSC preservation yielded results comparable to 24-hour SCS, quadrupling the functional preservation window.

    Conclusions:

    • DSC is a paradigm-shifting advancement in renal preservation, extending viable storage duration fourfold.
    • DSC improves graft quality compared to SCS.
    • The technique's simplicity makes it a clinically translatable solution to increase donor organ utilization.