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The crucial role of CFAS in maintaining acid-stress homeostasis of Hypsizygus marmoreus

  • 0National Engineering Research Center of Edible Fungi, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; College of Food Science and Engineering, Jiangsu Ocean University, Lianyungang 222005, China.
Ecotoxicology and Environmental Safety +

|

November 6, 2025

|

November 6, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

Hypsizygus marmoreus, an edible fungus, can maintain pH balance in acidic soils. Its cyclopropane-fatty-acyl-phospholipid synthase (CFAS) protein enhances acid stress tolerance and aids in soil bioremediation.

Area Of Science

  • Mycology
  • Environmental Science
  • Biochemistry

Background

  • Soil pH is crucial for soil quality, especially in acidic conditions.
  • Hypsizygus marmoreus demonstrates remarkable acid stress homeostasis.
  • Understanding the mechanisms of acid tolerance in H. marmoreus is vital for soil bioremediation.

Purpose Of The Study

  • To investigate the role of Hypsizygus marmoreus in maintaining acid stress homeostasis.
  • To elucidate the molecular mechanisms underlying its acid tolerance.
  • To explore the potential of H. marmoreus for acidic soil bioremediation.

Main Methods

  • Cultivation of H. marmoreus under varying citric acid concentrations.
  • Heterologous expression of H. marmoreus cyclopropane-fatty-acyl-phospholipid synthase (CFAS) in yeast.
  • Analysis of metabolic shifts, protein metabolism, and lipid metabolism.
  • Physiological indicator detection (ATP, ATPase activity, MDA, ROS) and transcriptomics.

Main Results

  • Low citric acid concentrations promote mycelial growth via protein metabolism.
  • High citric acid concentrations induce a metabolic shift towards soluble protein production, enhanced by lipid metabolism.
  • CFAS expression in yeast improved tolerance to citric acid and cadmium stress.
  • CFAS expression increased ATP, mitochondrial H+-ATPase activity, soluble protein, and enhanced lipid and mitochondrial energy metabolism, while reducing oxidative stress markers.

Conclusions

  • H. marmoreus possesses robust mechanisms for acid stress homeostasis.
  • CFAS plays a significant role in conferring acid and heavy metal stress tolerance.
  • H. marmoreus mycelia show potential for bioremediation of acidic soils.

Keywords:

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