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Updated: Jan 12, 2026

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
Published on: July 17, 2018
KLAK peptide in anticancer therapy: Achieving cancer cell apoptosis via mitochondrial membrane disruption using
1Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského Nám. 2, Prague 6, 162 00, Czech Republic.
Abstract:
An amphiphilic α-helical peptide called KLAK (sequence KLAKLAKKLAKLAK) possesses antimicrobial and anticancer activity. KLAK peptide disrupts bacterial membrane as well as eukaryotic mitochondrial membrane, which makes it an interesting tool for killing cancer cells. Nevertheless, the peptide itself suffers from inappropriate distribution in the body, low cell internalization ability causing its insufficient anticancer efficacy and potential toxicity toward the healthy part of the organism. This review focuses on the strategies, which have been widely employed to overcome this obstacle and reach mitochondria destruction and thus enhance anticancer effect in vitro and in vivo in various types of tumors. Various approaches to targeting KLAK towards cancer cells are discussed, such as peptide-based homing domains or amino acid modifications.
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