14,15-dihydroclerodin (C₂₄H₃₆O₇) induced cell cycle delay, antimitotic and cytotoxic effects in Allium cepa L. root...

14,15-Dihydroclerodin, or dihydroclerodin-I (DCLR), is a natural product that can also be synthesized through catalytic hydrogenation of clerodin. In this study, dihydroclerodin, purified from Clerodendrum viscosum leaves, was evaluated for its cell cycle arrest and cytotoxic effects in Allium cepa root tip cells and compared with colchicine-induced antimitotic activity. Treatments with dihydroclerodin at concentrations of 100, 200, and 300 µg mL^-1, and colchicine at 150 µg mL^-1 (as a positive control), were administered for 2, 4, and 4 + 16 h (4-hour treatment followed by 16 h of recovery). The highest frequency of metaphase cells was observed with dihydroclerodin (300 µg mL^-1) and colchicine (150 µg mL^-1) at 4 h, with values of 75.09 ± 0.93% and 78.31 ± 0.49%, respectively. Both treatments significantly increased the percentages of aberrant cells, chromosomal aberrations (CA), micronuclei (MN), C-metaphases (C-Met), and polyploid (PP) cells. These findings suggest that dihydroclerodin exhibits strong antimitotic effects, warranting further investigation of its potential in cancer chemotherapy due to its ability to induce cell cycle delay and cytotoxicity.