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Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Jan 12, 2026

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Published on: March 27, 2020

Fluorescence lifetime clocks quantify senescence and aging.

Chenxu Yan¹, Caiqi Liu¹, Bofang Liu²

¹Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, Institute of Fine Chemicals, Frontiers Science Center for Materiobiology and Dynamic Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.

|November 6, 2025

Summary

This summary is machine-generated.

Researchers developed a new method using fluorescent dyes to visually track aging in real-time within living organisms. This innovative approach offers a non-invasive way to measure biological age and cellular senescence.

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Last Updated: Jan 12, 2026

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Published on: March 27, 2020

Autofluorescence Imaging to Evaluate Cellular Metabolism

Autofluorescence Imaging to Evaluate Cellular Metabolism

Published on: November 15, 2021

Fluorescence Lifetime Macro Imager for Biomedical Applications

Fluorescence Lifetime Macro Imager for Biomedical Applications

Published on: April 7, 2023

Area of Science:

Aging biology
Molecular biology
Biophysics

Background:

Epigenetic and omics-based clocks are crucial for quantifying aging but lack real-time, in vivo applications.
Nucleolar ribosomal DNA methylation changes correlate with aging and cellular senescence.
Existing methods require extensive sample preparation, limiting real-time analysis.

Purpose of the Study:

To develop an image-based clock for real-time, in vivo age quantification.
To leverage shifts in rRNA species for age assessment.
To create a non-invasive method for measuring biological age and senescence.

Main Methods:

Engineered sensitive and photostable hybrid polymethine dyes selective for rRNA.
Developed a fluorescence lifetime imaging (FLIm) strategy.
Applied FLIm to quantify age- and senescence-dependent nucleolar RNA changes.

Main Results:

Demonstrated the ability to generate image-based clocks using selective rRNA dyes.
Achieved real-time, in vivo age quantification without extensive sample preparation.
Showcased translatability across cellular, tissue, and organismal scales (C. elegans, mice, human samples).

Conclusions:

Fluorescence lifetime imaging of rRNA provides a novel, in vivo method for measuring aging and senescence.
This strategy expands the research toolbox for aging biology and translational studies.
Enables direct visualization and quantification of age-related molecular changes in living systems.