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Updated: Jan 12, 2026
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Expression Significance of Serum Circular RNA CDYL and Circular RNA ROBO2 in Patients with Acute Myocardial
Fang Wang1, Rui Liu1, Cuirong Zhang1
1Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, People's Republic of China.
Insights
Serum circular RNAs CDYL and ROBO2 show potential for predicting major adverse cardiovascular events (MACE) in acute myocardial infarction (AMI) patients post-PCI. Combined detection offers significant predictive value for MACE risk.
Area of Science:
- Cardiovascular Research
- Molecular Biology
- Biomarker Discovery
Background:
- Acute myocardial infarction (AMI) remains a leading cause of mortality worldwide.
- Percutaneous coronary intervention (PCI) is a common treatment for AMI, but residual risk of major adverse cardiovascular events (MACE) persists.
- Identifying reliable biomarkers for MACE prediction is crucial for improving patient outcomes.
Purpose of the Study:
- To investigate serum circular RNA (circRNA) CDYL and circRNA ROBO2 expression in AMI patients.
- To explore the association between these circRNAs and MACE occurrence after PCI.
- To evaluate the clinical utility of combined circRNA CDYL and ROBO2 detection for MACE prediction.
Main Methods:
- A retrospective cohort study included 119 AMI patients undergoing PCI and 100 healthy controls.
- Serum circRNA CDYL and ROBO2 levels were quantified using quantitative real-time polymerase chain reaction (qRT-PCR).
- Statistical analyses included correlation, multivariate logistic regression, and receiver operating characteristic (ROC) curve analysis.
Main Results:
- Serum circRNA CDYL was downregulated, while circRNA ROBO2 was upregulated in AMI patients compared to controls.
- Lower circRNA CDYL and higher circRNA ROBO2 levels were observed in patients who experienced MACE post-PCI.
- CircRNA ROBO2 was an independent risk factor, and circRNA CDYL a protective factor for MACE.
- Combined detection yielded an AUC of 0.901 for MACE prediction, with high sensitivity and specificity.
Conclusions:
- Serum circRNA CDYL and ROBO2 levels are significantly associated with MACE risk in AMI patients after PCI.
- The combined detection of circRNA CDYL and ROBO2 demonstrates promising predictive value for MACE.
- Further prospective studies and mechanistic investigations are warranted to validate these findings.
Objective:
To investigate the expression changes of circular RNAs CDYL and ROBO2 in the serum of patients with acute myocardial infarction (AMI), explore their potential association with the occurrence of major adverse cardiovascular events (MACE), and evaluate the clinical value of their combined detection in predicting MACE after percutaneous coronary intervention (PCI).
Methods:
A retrospective cohort study was conducted on 119 AMI patients who underwent PCI (observation group) between February 2023 and February 2024. Based on post-PCI MACE occurrence, patients were categorized into Group A (with MACE, n=47) and Group B (without MACE, n=72). Additionally, 100 healthy volunteers were enrolled as controls. Serum levels of circRNA CDYL and circRNA ROBO2 were measured by quantitative real-time polymerase chain reaction (qRT-PCR). Pearson correlation analysis assessed their relationship. Multivariate logistic regression identified factors independently associated with MACE. Receiver operating characteristic (ROC) curves evaluated the predictive performance of each circRNA and their combination for MACE.
Results:
Serum circRNA CDYL was significantly downregulated in AMI patients, while circRNA ROBO2 was upregulated, showing a strong negative correlation (r = -0.659, P<0.001). The MACE group exhibited lower circRNA CDYL and higher circRNA ROBO2 levels than the non-MACE group (P<0.05). Multivariate analysis identified high circRNA ROBO2 as an independent risk factor for MACE, while high circRNA CDYL was a protective factor. The combined detection of both circRNAs predicted MACE with an area under the curve (AUC) of 0.901, significantly outperforming either marker alone (sensitivity=89.74%, specificity=80.51%).
Conclusion:
Serum circRNA CDYL and ROBO2 are significantly associated with MACE risk in AMI patients after PCI. Their combined detection shows promising predictive value. However, these findings from a retrospective study are preliminary and require validation in prospective cohorts and further mechanistic investigation.
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