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Published on: March 10, 2015
LGG/LAC-MMT combination mitigates AFB1-induced liver and intestinal injury in mice based on intestinal microbiota
Jiaxin Cheng1, Ying Gao1, Hongming Lv1
1College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing High-Tech Industrial Development Zone, Daqing, China.
Abstract:
AFB1 induces hepatotoxicity and enterotoxicity. Lactobacillus acidophilus (LAC) and Lactobacillus rhamnosus (LGG), both belonging to LAB, have strong binding affinity for AFB1. Montmorillonite (MMT) not only adsorbs AFB1 but also serves as a carrier for LAB, thereby enhancing their colonization ability and prolonging their survival. Despite the unclear effects of LGG/LAC-MMT combination on AFB1-induced tissue injury and intestinal microbiota disruption, this study aimed to determine whether it could effectively alleviate tissue damage from AFB1 exposure and enhance LAB colonization capacity in mouse intestines. Separately, LGG (2 × 109 cfu/mL) and LAC (2 × 109 cfu/mL) were combined with MMT (0.5 mg/kg), and the AFB1-intoxicated mice were gavaged with the mixtures for 4 weeks. Findings suggested that LGG, LAC, and MMT supplementation restored oxidative stress and inflammatory caused by AFB1 to some degree. Furthermore, they altered the intestinal microbiota structure, enhancing the colonization ability of LABs, thereby alleviating liver and intestinal injury. The combination of LGG/LAC-MMT was more effective, especially LAC-MMT. Overall, LGG/LAC-MMT exhibits a synergistic effect and can effectively ameliorate AFB1-induced tissue injury and intestinal microbiota disorder.
Insights
This study shows that combining Lactobacillus acidophilus (LAC) and Lactobacillus rhamnosus (LGG) with Montmorillonite (MMT) effectively reduces aflatoxin B1 (AFB1) liver and intestinal damage. The LAC-MMT combination was particularly effective in restoring gut health.
Area of Science:
- Toxicology
- Microbiology
- Gastroenterology
Background:
- Aflatoxin B1 (AFB1) causes significant liver and intestinal damage.
- Lactobacillus acidophilus (LAC) and Lactobacillus rhamnosus (LGG) bind AFB1, while Montmorillonite (MMT) adsorbs AFB1 and supports LAB.
- The combined effects of LGG/LAC-MMT on AFB1-induced injury and gut microbiota were unclear.
Purpose of the Study:
- To evaluate the efficacy of LGG/LAC-MMT in alleviating AFB1-induced tissue damage.
- To assess the enhancement of LAB colonization capacity in mouse intestines by LGG/LAC-MMT.
- To investigate the impact on AFB1-induced intestinal microbiota disruption.
Main Methods:
- AFB1-intoxicated mice were gavaged with LGG (2 × 10^9 cfu/mL) and LAC (2 × 10^9 cfu/mL) combined with MMT (0.5 mg/kg) for 4 weeks.
- Assessed restoration of oxidative stress and inflammation.
- Analyzed changes in intestinal microbiota structure and LAB colonization.
Main Results:
- LGG, LAC, and MMT supplementation partially restored oxidative stress and inflammation caused by AFB1.
- The treatments altered intestinal microbiota, enhancing LAB colonization and alleviating liver and intestinal injury.
- The combination of LGG/LAC-MMT showed synergistic effects, with LAC-MMT being particularly effective.
Conclusions:
- LGG/LAC-MMT synergistically ameliorates AFB1-induced tissue injury and intestinal microbiota disorder.
- The combination enhances LAB colonization, offering a potential therapeutic strategy against AFB1 toxicity.
- LAC-MMT demonstrated significant efficacy in mitigating AFB1-induced damage.
