Hemorheology Reference Intervals in Healthy Dogs Using the MIZAR Analyzer

Area of Science:

• Veterinary Hematology
• Biophysics
• Diagnostic Medicine

Background:

• Hemorheology, the study of blood flow properties, is underutilized in veterinary medicine.
• Red blood cell (RBC) rheology assessment offers potential for diagnosing and monitoring canine diseases.
• Establishing species-specific reference intervals (RIs) is crucial for clinical application.

Purpose of the Study:

• To determine hemorheological reference intervals (RIs) in healthy dogs.
• To compare RIs generated at 620 nm versus 800 nm wavelengths.
• To evaluate the impact of adhering to ASVCP guidelines on RI generation.

Main Methods:

• Collected whole blood from 42 healthy dogs in EDTA tubes.
• Analyzed 13 hemorheological parameters using the MIZAR Analyzer.
• Calculated RIs using parametric/non-parametric methods, with and without ASVCP guideline adherence, at 620 nm and 800 nm.

Main Results:

• Established RIs for 13 hemorheological parameters at both wavelengths.
• Observed minimal differences for 5/13 parameters between wavelengths; others shifted higher at 800 nm.
• Found ASVCP guideline adherence critical for 6/13 parameters, yielding clinically unsuitable results otherwise.

Conclusions:

• Defined specific RIs for canine hemorheology at 620 nm and 800 nm.
• Emphasized the necessity of following ASVCP guidelines for reliable veterinary hemorheology assessments.