Related Experiment Video

Updated: Jan 12, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
07:16

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

20.6K

WTAP promotes the proliferation of multiple myeloma by regulating the hippo pathway through m6A modification of

Han Xu1, Manting Xu1, Jiamin Ding1

  • 1Department of Hematology, the First Affiliated Hospital of Anhui Medical University, Hefei, China.

Leukemia & Lymphoma
|November 7, 2025
PubMed

Insights

Wilms

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Multiple myeloma (MM) is an incurable blood cancer with limited therapeutic targets.
  • Wilms' tumor 1-associated protein (WTAP) is implicated in MM, but its role is not fully understood.

Purpose of the Study:

  • To investigate the molecular mechanisms of WTAP in multiple myeloma progression.
  • To evaluate WTAP as a potential therapeutic target for MM.

Main Methods:

  • Analysis of bone marrow samples from newly diagnosed MM patients.
  • Quantitative real-time PCR (qRT-PCR) and Western blot for WTAP expression.
  • EdU and flow cytometry for cell proliferation; RNA immunoprecipitation (RIP) for protein interactions.

Main Results:

  • WTAP was overexpressed in MM patients and linked to poorer survival.
  • WTAP knockdown inhibited MM cell proliferation and inflammation.
  • MAP6D1 identified as a WTAP target, suggesting Hippo pathway involvement.

Conclusions:

  • WTAP promotes MM proliferation by targeting MAP6D1 and regulating the Hippo signaling pathway.
  • WTAP represents a promising therapeutic target for multiple myeloma.
Keywords:
MAP6D1Multiple myelomaWTAPhippo pathwaytumorigenesis

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