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Related Concept Videos

Hand hygiene01:23

Hand hygiene

Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
Retarders01:19

Retarders

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Superplasticizers01:30

Superplasticizers

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Chemical Agents for Microbial Control01:27

Chemical Agents for Microbial Control

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Bioreactor Controls-I

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Engineering a Bilayered Hydrogel to Control ASC Differentiation
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One-Step Tunable Human Hair Keratin Gradient Hydrogel with Antibacterial Activity for Tissue Engineering.

Marin Zhen Lin Yee1,2, Yun Wei Lim1, Logeshwari Muthualagu Natarajan1

  • 1School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.

Small (Weinheim an Der Bergstrasse, Germany)
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Summary

Researchers developed a novel gradient hydrogel using silver ions and human hair keratin for tissue engineering. This biomimetic material promotes wound healing and exhibits antimicrobial properties, offering a sustainable solution.

Keywords:
antibacterialgradient hydrogelhuman hair keratinsmetal‐thiolate complexationtissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Gradient hydrogels mimic native tissue heterogeneity, crucial for effective tissue engineering.
  • Metal-thiolate complexation offers a versatile mechanism for hydrogel fabrication.
  • Silver ions (Ag+) provide antimicrobial properties, beneficial for wound healing applications.

Purpose of the Study:

  • To develop a novel gradient hydrogel using a metal-thiolate complexation mechanism.
  • To investigate the properties and efficacy of silver-loaded human hair keratin (HHK) gradient hydrogels.
  • To evaluate the potential of these hydrogels in a wound healing model.

Main Methods:

  • Fabrication of gradient hydrogels via diffusion of Ag+ into HHK solution.
  • Characterization of gelation kinetics, physical, mechanical, and biochemical properties.
  • Assessment of antibacterial activity using disk diffusion tests against Staphylococcus aureus.
  • In vitro evaluation of human dermal fibroblast (HDF) viability, proliferation, and function.
  • In vivo assessment in a full-thickness wound healing mouse model.

Main Results:

  • A novel, one-step gradient hydrogel was successfully produced using Ag+ and HHK.
  • The hydrogel exhibited a porosity gradient and dissimilar surface morphologies.
  • Significant antibacterial activity against Staphylococcus aureus was confirmed.
  • Hydrogels supported high HDF viability and proliferation, comparable to collagen.
  • In vivo studies showed enhanced reepithelialization and collagen deposition compared to GelMA.

Conclusions:

  • Metal-thiolate complexation is a practical approach for creating biomimetic gradient hydrogels.
  • The developed Ag-HHK gradient hydrogel demonstrates potential for tissue regeneration and wound healing.
  • This sustainable material combines desirable mechanical, biochemical, and antimicrobial properties.