A Multicomponent Salmon PDRN/Sodium Alginate/Gelatin Hydrogel Promotes Infected Wound Repair in Rat

Infected wound healing is a clinical problem owing to excessive inflammatory responses. In this study, a multicomponent hydrogel GOP was prepared by employing salmon polydeoxyribonucleotide (PDRN), oxidized sodium alginate (OSA), and gelatin (Gel) as starting materials. The hydrogel with three-dimensional structure was synthesized through the Schiff-base cross-linking of PDRN, Gel, and OSA. Hydrogen bonding and electrostatic interaction also participated in the formation of the three-dimensional network. Hydrogel GOP demonstrated a good mechanical property and tissue adhesion ability. In vitro studies revealed the antibacterial activity of hydrogel GOP toward S. aureus and E. coli, along with remarkable antioxidant activity. Biocompatibility tests showed high cell viability (>80%) and good ability to promote cell migration. Hemolysis assay revealed minimal hemotoxicity (<2.1%). Using a murine full-thickness dermal injury model, GOP treatment achieved small residual wound area (2.3%) on day 14. Histological analyses demonstrated reduced inflammation cells and enhanced collagen deposition. Immunofluorescence analyses showed suppressed inflammation level (increased IL-10) and improved angiogenesis (upregulated CD31 and α-SMA). These results revealed that hydrogel GOP represented a promising multifunctional dressing for infected wound management.