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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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The Antiviral System of Bacteria and Archaea: CRISPR01:23

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CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Highly Sensitive Field Detection Technology for Anthrax Based on the CRISPR/Cas13a System.

Jiao Fan1,2, Luyao Huang1,2, Jiahui Chen1,2

  • 1Chinese PLA Center for Disease Control and Prevention, Beijing, China.

Microbial Biotechnology
|November 7, 2025
PubMed
Summary
This summary is machine-generated.

We developed a rapid, highly sensitive CRISPR/Cas13a assay for detecting Bacillus anthracis on-site. This portable point-of-care technology provides accurate results within 30 minutes, crucial for infectious disease surveillance.

Keywords:
Bacillus anthracisCRISPR/Cas13anucleic acid detectionpoint‐of‐care testing

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Bacillus anthracis poses a significant bioterrorism threat.
  • Rapid and sensitive detection methods are crucial for timely response.
  • Existing detection methods may lack portability or speed for on-site applications.

Purpose of the Study:

  • To develop a highly sensitive and specific on-site detection technology for Bacillus anthracis.
  • To integrate Multiple Enzyme Isothermal Rapid Amplification (MIRA) with CRISPR/Cas13a.
  • To establish a portable point-of-care testing (POCT) device for B. anthracis.

Main Methods:

  • Integration of MIRA with a CRISPR/Cas13a detection system.
  • Optimization of crRNA selection, MIRA primers, and reaction conditions.
  • Development of a CRISPR-based point-of-care testing device with lyophilized reagents.

Main Results:

  • The optimized CRISPR/Cas13a assay achieved a detection limit of 1000 copies/mL for B. anthracis.
  • The developed POCT device demonstrated a detection limit of 250 copies/mL within 30 minutes.
  • The assay showed high specificity against common respiratory pathogens and high accuracy in simulated clinical samples.

Conclusions:

  • A highly sensitive and portable CRISPR/Cas13a-based technology for on-site B. anthracis detection was successfully established.
  • The developed POCT device offers rapid, accurate, and specific detection capabilities.
  • This technology holds significant potential for the surveillance of emerging infectious diseases.