Related Experiment Video
Updated: Jan 12, 2026

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
Published on: February 7, 2019
Cross-platform motif discovery and benchmarking to explore binding specificities of poorly studied human
Ilya E Vorontsov1, Ivan Kozin2,3, Sergey Abramov1,4
1Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russia.
Abstract:
A sequence motif representing the DNA-binding specificity of a transcription factor (TF) is commonly modelled with a positional weight matrix (PWM). Focusing on understudied human TFs, we processed results of 4,237 experiments for 394 TFs, assayed using five different experimental platforms. By human curation, we approved a subset of experiments that yielded consistent motifs across platforms and replicates, and evaluated quantitatively the cross-platform performance of PWMs obtained with ten motif discovery tools. Notably, nucleotide composition and information content are not correlated with motif performance and do not help in detecting underperformers, while motifs with low information content, in many cases, describe well the binding specificity assessed across different experimental platforms. By combining multiple PMWs into a random forest, we demonstrate the potential of accounting for multiple modes of TF binding. Finally, we present the Codebook Motif Explorer ( https://mex.autosome.org ), cataloguing motifs, benchmarking results, and the underlying experimental data.
More Related Videos
Related Concept Videos
Cooperative Binding of Transcription Regulators
Cooperative Binding of Transcription Regulators
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Transcription Factors
General Transcription Factors
Co-activators and Co-repressors

