Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Tumor microenvironment-specific nanomedicine: from biology-driven to multi-omics-guided precision engineering.

Journal of hematology & oncology·2026

Same author

Spatial multi-omics reveals region-specific molecular signatures in a 6-OHDA model of Parkinson's disease.

NPJ Parkinson's disease·2026

Same author

A Prodrug Approach for Activity-Based Chemical Modulation toward Multiple Pathological Targets in Alzheimer's Disease.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Wafer-Scale 2D High-Entropy Transition Metal Dichalcogenide Thin-Film Catalysts for Efficient and Durable Photoelectrochemical Hydrogen Production.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Two-dimensional hard masks push three-dimensional patterning.

Nature materials·2026

Same author

TBK1-NQO1 negative feedback loop regulates antiviral innate immunity.

Cell communication and signaling : CCS·2026

Search research articles

Related Experiment Video

Updated: Jan 12, 2026

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Published on: October 17, 2018

Humulus japonicus Water Extract Inhibits Neuroinflammation Through the p38 MAPK Signaling Pathway in a Systemic

Ju-Eun Kim¹, Jun Go¹, Hye-Yeon Park¹

¹Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.

Journal of Medicinal Food

|November 8, 2025

Summary

Humulus japonicus extract (HJW) reduces neuroinflammation by inhibiting key inflammatory markers and suppressing microglial activation. This natural compound may improve cognitive function and memory in neurodegenerative conditions.

Keywords:

BV-2 microglial cell Iba-1 MAPK signaling novel object recognition test proinflammatory cytokines

More Related Videos

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Published on: August 23, 2022

Related Experiment Videos

Last Updated: Jan 12, 2026

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Sub-acute Cerebral Microhemorrhages Induced by Lipopolysaccharide Injection in Rats

Published on: October 17, 2018

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Published on: June 14, 2020

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Published on: August 23, 2022

Area of Science:

Neuroscience
Immunology
Pharmacology

Background:

Neuroinflammation, an immune response in the central nervous system, is implicated in neurodegenerative diseases.
Microglia are key immune cells in the brain, and their activation contributes to neuroinflammation.

Purpose of the Study:

To investigate the effects of Humulus japonicus water extract (HJW) on neuroinflammation.
To elucidate the mechanisms underlying HJW's effects in lipopolysaccharide (LPS)-induced models.

Main Methods:

In vitro studies using lipopolysaccharide (LPS)-treated BV-2 murine microglial cells.
In vivo studies using a mouse model with LPS administration.
Assessed nitric oxide, inflammatory cytokines (IL-6, TNF-α), gene expression (Il1b, Il6), microglial activation, phospho-p38 levels, and cognitive function (novel object recognition test).

Main Results:

HJW inhibited LPS-induced secretion of nitric oxide, IL-6, and TNF-α, and reduced Il1b mRNA expression in BV-2 cells.
In vivo, HJW downregulated Il6 expression in the cerebral cortex and reduced TNF-α and IL-6 in blood.
HJW suppressed microglial activation, reduced phospho-p38 levels, and alleviated LPS-induced cognitive deficits.

Conclusions:

Humulus japonicus water extract (HJW) demonstrates significant anti-neuroinflammatory effects.
HJW may serve as a therapeutic natural extract for neuroinflammation, potentially enhancing memory and cognitive functions.