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POSTN+ Cancer-Associated Fibroblasts Promote Gastric Cancer Invasion by Activating AKT Signaling.

Xiuxiu Xu1, Tingting You1, Zhao Sun1

  • 1Department of Medical Oncology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.

Stem Cells and Development
|November 8, 2025
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Cancer-associated fibroblasts secreting POSTN promote gastric cancer invasion. Targeting POSTN+ CAFs and the AKT pathway may inhibit metastasis and improve patient survival.

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POSTNcancer-associated fibroblastgastric cancerinvasion

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Area of Science:

  • Oncology
  • Cancer Biology
  • Cell Biology

Background:

  • Metastasis is a leading cause of cancer mortality.
  • Cancer-associated fibroblasts (CAFs) significantly influence tumor progression.
  • A POSTN-secreting CAF subpopulation is linked to gastric cancer (GC) invasion and survival.

Purpose of the Study:

  • To investigate the role of POSTN-expressing CAFs in gastric cancer (GC) invasion.
  • To elucidate the underlying molecular mechanisms of POSTN-mediated GC cell migration and invasion.

Main Methods:

  • Analysis of The Cancer Genome Atlas (TCGA) data for POSTN in GC.
  • In vitro induction of CAFs from mesenchymal stem cells (MSCs) using GC exosomes.
  • Lentiviral manipulation of POSTN expression in CAFs.
  • In vivo validation in a nude mouse GC model.
  • Assessment of integrin and AKT signaling pathways.

Main Results:

  • TCGA analysis revealed POSTN's association with epithelial-mesenchymal transition and extracellular matrix degradation pathways in GC.
  • GC exosomes induced POSTN expression in MSC-derived CAFs.
  • POSTN overexpression in CAFs enhanced GC cell migration and invasion, while knockdown reduced these effects.
  • POSTN+ CAFs activated integrin-dependent AKT signaling, promoting GC invasion.
  • AKT inhibition abrogated the pro-migratory and invasive effects of POSTN-overexpressing CAFs.

Conclusions:

  • POSTN-expressing CAFs are key drivers of gastric cancer invasion.
  • The POSTN-CAFs-AKT signaling axis promotes GC cell migration and invasion.
  • Targeting POSTN+ CAFs or the AKT pathway presents a potential therapeutic strategy for advanced gastric cancer.