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Molecular methods for high-throughput, multiplexed, and automated genome editing in prokaryotes and eukaryotes
Dominic Kösters1, Jan Marienhagen1
1Institute of Bio and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, D-52425 Jülich, Germany; Institute of Biotechnology, RWTH Aachen University, Worringer Weg 3, D-52074 Aachen, Germany.
Current Opinion in Biotechnology
|November 8, 2025
Summary
Advancements in genome engineering, including DNA editing and lab automation, enable high-throughput, multiplexed strain engineering. This review covers new technologies for automated genome modification in prokaryotic and eukaryotic organisms.
Area of Science:
- Synthetic Biology
- Genomics
- Molecular Biology
Background:
- Genome engineering is vital for advancing strain engineering and synthetic biology.
- DNA editing techniques have evolved, allowing for higher throughput and simultaneous genome modifications.
- Laboratory automation is increasingly accessible, facilitating large-scale genome editing.
Purpose of the Study:
- To review recent developments in high-throughput, multiplexed, and automated strain engineering technologies.
- To highlight the role of robot-assisted platforms in modern molecular biology.
- To discuss applications in both prokaryotic and eukaryotic organisms.
Main Methods:
- Review of recent literature on genome engineering technologies.
- Focus on high-throughput, multiplexed, and automated approaches.
- Examination of applications in prokaryotic and eukaryotic systems.
Main Results:
- Significant progress has been made in automated and high-throughput genome engineering.
- Multiplexed genome modifications are now feasible at scale.
- Robot-assisted platforms are enhancing the efficiency of strain engineering.
Conclusions:
- Automated, high-throughput, and multiplexed genome engineering are transforming synthetic biology.
- These technologies are essential for fully realizing the potential of modern molecular biology tools.
- Continued development is expected to accelerate biological research and applications.
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