Selective enrichment for genetic markers in DNA released by competent cultures of Bacillus subtilis

Deoxyribonucleic acid is released into the growth medium by Bacillus subtilis at the time of competence. This DNA is enriched for the genetic markers which have previously been demonstrated to be elevated in membrane-DNA preparations and more recently in cell wall-DNA complexes. Furthermore, the purA16/leu-8 relative marker enrichment varies with time, reaching its highest point at the time of maximal competence. Enrichment remains elevated for at least 60 min further in the competence regimen. Thr results suggest that certain genetic markers of the B. subtilis chromosome are preferentially more available to the external medium as the development of competence proceeds.