Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

5.4K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
5.4K
Membrane Fluidity01:23

Membrane Fluidity

172.3K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
172.3K
Membrane Fluidity01:26

Membrane Fluidity

14.4K
Membrane fluidity is explained by the fluid mosaic model of the cell membrane, which describes the plasma membrane structure as a mosaic of components—including phospholipids, cholesterol, proteins, and carbohydrates—that gives the membrane a fluid character.
Mosaic nature of the membrane
The mosaic characteristic of the membrane helps the plasma membrane remain fluid. The integral proteins and lipids exist as separate but loosely-attached molecules in the membrane. The membrane is...
14.4K
Multi-pass Transmembrane Proteins and β-barrels01:09

Multi-pass Transmembrane Proteins and β-barrels

6.4K
In multi-pass transmembrane proteins, the polypeptide chain crosses the membrane more than once. The transmembrane polypeptide chain either forms an α-helix or β-strand structure. α-Helix containing multi-pass transmembrane proteins are ubiquitous, whereas β-strand containing ones are mainly found in gram-negative bacteria, mitochondria, and chloroplasts.
α-Helix containing multi-pass transmembrane proteins
Multi-pass transmembrane proteins such as...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of ADSS2-mediated de novo AMP biosynthesis re-sensitizes acute myeloid leukemia to BH3 mimetics.

Nature cancer·2026
Same author

CholBindNet as an interpretable neural network for cholesterol-binding site classification.

Communications chemistry·2026
Same author

Evolutionary diversity and structural dynamics of the outer membrane protein Ail in <i>Yersinia</i>.

Journal of biomolecular structure & dynamics·2026
Same author

Prediction of bacterial protein-compound interactions with only positive samples.

Bioinformatics (Oxford, England)·2026
Same author

A two-step clockwork mechanism opens a proteo-lipidic pore in PIEZO2.

Nature chemical biology·2026
Same author

Dynamic nature of Staphylococcus aureus type I signal peptidases.

Biophysical journal·2025

Related Experiment Video

Updated: Jan 12, 2026

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.1K

Optimizing a coarse-grained model for large-scale membrane protein simulation.

Chen Yun Wen1, Yun Lyna Luo1, Jesper J Madsen2

  • 1Department of Biotechnology and Pharmaceutical Sciences, College of Pharmacy, Western University of Health Sciences, Pomona, California.

Biophysical Reports
|November 8, 2025
PubMed
Summary
This summary is machine-generated.

We optimized a coarse-grained (CG) lipid model for large-scale membrane simulations. The improved model enhances stability, enabling studies of membrane protein-induced bilayer deformations at physiologically relevant scales.

More Related Videos

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.4K
Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K

Related Experiment Videos

Last Updated: Jan 12, 2026

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches
07:31

Author Spotlight: Advancing Cell Membrane Biophysics - Exploring Interactions and Challenges Through Experimental and Computational Approaches

Published on: September 1, 2023

3.1K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.4K
Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K

Area of Science:

  • Biophysics
  • Computational Biology
  • Materials Science

Background:

  • Coarse-grained (CG) models are essential for simulating membrane proteins at large scales.
  • Simulating long-range bilayer deformations induced by membrane proteins at submicrometer scales is computationally challenging.
  • Existing generic solvent-free CG lipid models face limitations in stability for large membrane systems.

Purpose of the Study:

  • To assess and optimize a generic solvent-free CG lipid model for large-scale molecular dynamics simulations of membrane proteins.
  • To overcome the instability issues (membrane poration, unphysical undulations) observed in previous CG lipid models beyond a critical membrane size.
  • To enable the investigation of bilayer deformations induced by membrane proteins like PIEZO in systems of varying mechanical properties.

Main Methods:

  • Systematic optimization of a generic solvent-free coarse-grained (CG) lipid model.
  • Large-scale molecular dynamics simulations of membrane systems.
  • Assessment of model stability with increasing membrane size.
  • Simulation of membrane deformation induced by the mechanosensitive ion channel PIEZO.

Main Results:

  • The generic CG lipid model exhibited instability (poration, undulations) beyond a critical membrane size.
  • Systematic optimization significantly improved the model's stability for larger membrane systems.
  • The optimized CG model successfully simulated membrane deformations induced by PIEZO channels in bilayers with tunable mechanical properties.

Conclusions:

  • The optimized CG lipid model provides enhanced stability for large-scale membrane simulations.
  • This improved model facilitates the study of bilayer-mediated membrane protein interactions.
  • The model bridges the gap between continuum elasticity theory and atomistic simulations for membrane biophysics research.