Related Concept Videos
01:15Western Blotting
20.1K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
20.1K
02:57Southern Blot
22.3K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
22.3K
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Related Experiment Video
Updated: Jan 12, 2026
07:45
Western Blotting: Sample Preparation to Detection
Published on: October 14, 2010
145.2K
Brief guide to western blot assays.
Seokjun G Ha1, Ji-Hoon Park1, Mi-Young Kim1
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, South Korea.
Molecules and Cells
|November 8, 2025
Summary
This guide explains the Western blot technique, a key method for detecting specific proteins using antibodies. It covers the entire process, from antibody selection to data quantification, aiding researchers new to protein detection assays.
Area of Science:
- Biochemistry
- Molecular Biology
- Immunology
Background:
- Western blotting is a fundamental technique in molecular biology for identifying specific proteins.
Keywords:
Western blot
