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Updated: Jul 18, 2026
11:02
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
Published on: April 29, 2011
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Gpr101 expression during early stages of murine development
John R Wells1, Maria B Padua2, Stephanie M Ware2
1Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN, USA.
Gene Expression Patterns : GEP
|November 8, 2025
Summary
This study reveals GPR101 expression in embryonic mice, primarily in the central nervous system, contrasting with adult patterns and offering new insights into growth regulation.
Area of Science:
- Developmental biology
- Neuroscience
- Endocrinology
Background:
- G protein-coupled receptor 101 (GPR101) is linked to X-linked acrogigantism (X-LAG).
- GPR101 expression is known in adult tissues but poorly understood during embryonic development.
Purpose of the Study:
- To investigate the spatiotemporal expression patterns of Gpr101 during early mouse embryonic development (E7.5-E15.5).
Main Methods:
- Utilized a global Gpr101 knock-in mouse model (Gpr101tm1b(KOMP)Mbp) with a LacZ reporter.
- Analyzed LacZ expression to map Gpr101 gene activity.
Main Results:
- Gpr101 expression was detected starting at E10.5, localized near the telencephalic vesicle.
- Expression was predominantly observed in the central nervous system.
- Notably, Gpr101 expression was absent in the hypothalamus and pituitary gland during the assessed embryonic stages.
Conclusions:
- Gpr101 exhibits a distinct expression profile during embryonic development compared to adult tissues.
- These findings enhance our understanding of GPR101's role in early development and growth.
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