Optimization of Oxygen Supply Conditions for Hypothermic Oxygenated Machine Perfusion

Hypothermic oxygenated machine perfusion (MP) contributes to the reduction of delayed graft function and ischemic reperfusion injury in donated after circulatory death donors. In this study, we focused on perfusate temperature, oxygen consumption, and oxygen supply and proposed the possibility of assessing organ function. We also calculated the oxygen supply volume for membrane, pressure, dissolved, and bubble dissolved oxygen supply methods during MP, and summarized the optimal oxygen supply methods. The consumption of adult human kidney and liver based on body surface area was converted to 5.3, 1.6, 0.6, and 0.4 mL/min for human kidney, and 86.9, 31.9, 14.3, and 9.6 mL/min for human liver (37, 22, 10, and 4°C, respectively). The results suggest that a compact oxygenation method is sufficient to supply oxygen at temperatures below 10°C, the hypothermic oxygenated MP temperature range, and that new oxygenation techniques should be investigated.