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Related Concept Videos

Conjugated Proteins02:50

Conjugated Proteins

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Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
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Alternative In Vitro Methods for the Determination of Viral Capsid Structural Integrity
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Physiocochemical studies on L-cell virions.

J L Nichols, K Quade, R B Luftig

    Journal of Virology
    |March 1, 1973
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    Summary
    This summary is machine-generated.

    The L-cell virion (LCV) is a defective RNA tumor virus. It shares properties with infectious viruses but lacks tumor-inducing or cell-transforming capabilities due to negligible DNA polymerase activity.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Oncology

    Background:

    • Mouse L cells produce L-cell virions (LCVs) in culture.
    • LCVs share physical and biochemical characteristics with known RNA tumor viruses.

    Purpose of the Study:

    • To characterize the L-cell virion (LCV).
    • To investigate the potential oncogenic or transforming activity of LCV.
    • To explore the reasons for LCV's defective nature.

    Main Methods:

    • Purification of LCV from cell culture supernatant.
    • Density gradient centrifugation to determine virion density.
    • Electron microscopy for particle morphology.
    • SDS-PAGE for polypeptide analysis.
    • RNA isolation and characterization.
    • Assays for DNA polymerase activity.

    Main Results:

    • LCV purified with a density of 1.16 g/cm³.
    • Virions are spherical (146.7 nm) with surface knobs.
    • 15 polypeptides and three RNA species (80-88s, 7s, 4s) were identified.
    • LCV lacks tumor-inducing and cell-transforming abilities.
    • Negligible DNA polymerase activity was detected in LCV.

    Conclusions:

    • LCV is a defective variant of RNA tumor viruses.
    • Its defective nature is likely linked to its low DNA polymerase activity.
    • LCV serves as a model for studying viral defects and oncogenesis.